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Identification of IgE binding epitopes of peptide fragments of Bla g 6 from German cockroach (Blattella germanica)
DC Field | Value | Language |
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dc.contributor.author | 은선진 | - |
dc.date.accessioned | 2015-12-24T10:10:46Z | - |
dc.date.available | 2015-12-24T10:10:46Z | - |
dc.date.issued | 2009 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/137337 | - |
dc.description | Dept. of medical science/석사 | - |
dc.description.abstract | German cockroach allergens induce IgE-mediated hypersensitivity. Among the German cockroach allergens studied, Bla g 6 shows homology to muscle protein troponin C. It contains four calcium-binding domains at 20-30, 56-67, 96-107, and 132-143 amino acid (aa) residues, and its immunoglobulin E (IgE) reactivity is dependent upon calcium ion level. However, the IgE binding epitopes of Bla g 6 have not been studied. This study aimed to identify the IgE binding epitopes from the five linear peptide fragments of Bla g 6. The full-length and five peptide fragments (P1: aa 1-111, P2: aa 1-95, P3: aa 33-111, P4: aa 80-151, and P5: aa 33-151) of Bla g 6 were generated by polymerase chain reaction (PCR) and expressed in Escherichia coli (E. coli). Enzyme-linked immunosorbent assay (ELISA) was performed on 24 patients’ sera that adjusted the final concentration 10 mM of CaCl2 to determine the IgE activities of Bla g 6. Eight sera (33.3%), 9 sera (37.5%), and 11 sera (45.8%) showed IgE reactivity to Bla g 6.0101, Bla g 6.0201, and Bla g 6.0301, respectively. Among the sera from the positive IgE reactivity, three patients’ sera were selected and the IgE reactivity was measured by ELISA with the five recombinant peptide fragments of Bla g 6. Based on IgE responses, one patient’s serum exhibited the strongest IgE reactivity. I assumed that the aa between 96-151 residues, including the calcium binding domains III and IV, would be important for IgE binding. These results may provide information that will yield safe diagnostic methods and immunotherapeutics. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Identification of IgE binding epitopes of peptide fragments of Bla g 6 from German cockroach (Blattella germanica) | - |
dc.title.alternative | 독일바퀴(Blattella germanica)의 알레르겐, Bla g 6의 peptide 단편들의 IgE 반응 항원결정기(epitope)분석 | - |
dc.type | Thesis | - |
dc.contributor.alternativeName | Un, Sun Jin | - |
dc.type.local | Thesis | - |
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