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Association of serum phospholipid long-chain polyunsaturated fatty acids with cardiometabolic risk : case-control and experimental studies

Other Titles
 한국인에서 장쇄 다가 불포화지방산과 심혈관계질환위험과의 연관성 
Authors
 송주현 
Issue Date
2013
Description
Dept. of Science for Aging/박사
Abstract
Objective: This study aimed to investigate the association of serum phospholipid polyunsaturated fatty acids (PUFAs) with cardiometabolic risk in controls and coronary artery disease (CAD) patients including metabolic syndrome (MetS). Additionally, we tried to decipher the role of FAs on cardiovascular risk through experimental study.Methods: Serum phospholipid FA compositions and cardiovascular risk factors were measured in controls (non-MetS healthy: n=987, MetS: n=214) and CAD patients (non-MetS: n=152, MetS: n=56) aged from 30 to 69 years. Experimental assays i.e. a tetrazole (MTT) assay and a fluorescence imaging were additionally performed in vascular smooth muscle cells (VSMCs) with the treatment of selected PUFAs.Results: Biomarkers for cardiovascular risks were significantly different among the 4 subgroups (healthy control, MetS people, CAD only patients and CAD patients with MetS). HOMA-insulin resistance was significantly higher and LDL particle size was lower in MetS people, particularly in CAD patients carrying MetS compared with healthy people. High sensitivity C-reactive proteins were significantly higher and plasma adiponectins were lower in CAD patients, particularly in those carrying MetS than in healthy controls. Plasma LDL particle size were largest in healthy controls and lower in CAD patients, much lower in MetS people and lowest in CAD patients with MetS. Serum phospholipid FA compositions were also significantly different among the 4 groups. Linoleic acid (LA, C18:2-6) were highest in healthy control, lowest and highest in CAD patients carrying MetS. Dihomo--linolenic acids (DGLAs, C20:3-6) were lowest in healthy control and highest in CAD patients with MetS. Docosahexaenoic acid (DHA, C22:6-3) were lower, and arachidonic acid (AA, C20:4-6) and 6/3-PUFA were higher in CAD patients than in controls regardless of carrying MetS. Myristic acid (C14:0), and 3-polyunsaturated FA (3-PUFA) were significantly lower in CAD patients particularly in those carrying MetS than non-MetS healthy controls. The significances for these parameters maintained after adjusted for age, gender, cigarette smoking, alcohol consumption and total energy expenditure and total calorie intake. Multiple stepwise regression analysis revealed that AA and DHA among long-chain FAs (LC-FAs) mainly contributed to the gradual increased or decreased risk for MetS, CAD, and CAD with MetS (adjusted -coefficients for AA: 0.336, for DHA: -0.296) together with age, major MetS factors, LA, DGLA and gender (r=0.529, p<0.001). Additionally, experimental works shows that LC-FAs particularly DHA significantly suppress VSMC proliferation, which may participate in the signaling pathway for example, those of JNK, ERK and p38. DHA also seemed to be associated with the movement of p65 from cytosol to nucleus, which is closely associated with oxidative stress/inflammatory response.Conclusion Serum phospholipid FA compositions were significantly different according to health status. Among long-chain FAs, AA and DHA were main contributors to the gradual increased or decreased risk of MetS, CAD, and CAD with MetS. Particularly, DHA was closely associated with suppression of VSMC proliferation through signaling pathways and also participated in the cellular inflammatory and oxidative stress responses.
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Appears in Collections:
7. Others (기타) > Others (기타) > 5. Others
Yonsei Authors
Song, Ju Hyun(송주현)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/136312
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