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Cytokine responses related to mycobacterium tuberculosis infection status

Other Titles
 결핵균 감염형태에 따른 싸이토카인 반응 양상 
Authors
 김성현 
Issue Date
2013
Description
Dept. of Biomedical Laboratory Science/박사
Abstract
Tuberculosis (TB), mainly caused by Mycobacterium tuberculosis (MTB), remains a major infectious disease. According to a psy World Health Organization report, one-third of the world’s population is latently infected with MTB. Latently infected individuals have a potential risk of developing active disease in their lifetimes. Data from a TB natural psy history study show that in 5–10% of MTB-infected individuals, the infection progressed to active disease. In addition, the conversion rate from latent tuberculosis infection (LTBI) to active disease is greater in immunosuppressed individuals. Therefore, rapid diagnostic tests and effective treatment of LTBI are very important to reduce and control the TB burden. In this regard, investigating and understanding the mRNA expression levels of various immune markers in response to MTB-specific antigens in subjects with different MTB infection statuses are essential for the development of new immune-diagnostic assays. In this study, the mRNA expression levels of 8 immune markers (TH-1 type factors: IFN-γ, TNF-α, and IL-2R; TH-2 type cytokines: IL-4 and IL-10; and IFN-γ-induced chemokines: CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC)) in response to MTB-specific antigens and T-cell mitogen in different MTB infection statuses were investigated. In briefly, the results showed that individually, IL-2R and CXCL10 were found to be suitable markers for detecting MTB infection. On the other hand, TNF-α and CXCL9 was a suitable marker for distinguishing active TB disease from LTB. For optimal sensitivity, simultaneous detection of multiple targets was attempted, and the result was estimated using the sum of the mRNA expression of each gene. Among the numerous combinations of target genes, the combination of IFN-γ, TNF-α, and IL-2R showed the best performance for detecting MTB infection. The combination of TNF-α, IL-2R, CXCL9, and CXCL10 could detect MTB infection and that also could differentiate active TB disease from LTBI. Thus, a combination of suitable markers is more helpful for the efficient diagnosis of MTB infection. The results from this study indicate that in order to detect the MTB infection status, various immune markers which participate in the specific immune response to MTB infection may be useful. The assay developed in this study may be useful for follow-up study of TB close-contacts groups and for psy monitoring extra-pulmonary TB (EPTB) chemotherapy effect.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Others (기타) > 3. Dissertation
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/136258
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