FADS gene polymorphisms in Koreans association with ω-6 polyunsaturated fatty acids in serum phospholipids, lipid peroxidation and coronary artery disease

Abstract

[한글]

[영문]Objective: We investigated the association of FADS gene polymorphism in this region with PUFAs in serum phospholipids, lipid peroxides and coronary artery disease (CAD) in Koreans. Methods: CAD patients (n=756, 40-79 years) and healthy controls (n=890) were genotyped for rs174537 near FADS1 (FEN1 rs174537G>T), FADS2 (rs174575, rs2727270), and FADS3 (rs1000778) SNPs. The odds ratios (ORs) on CAD risk were calculated and serum PUFA composition and lipid peroxide variables were measured. Results: Among 4 SNPs, only rs174537G>T was different in the allele frequencies between controls and CAD patients after adjustment for age, BMI, cigarette smoking, alcohol consumption, systolic and diastolic blood pressure (P=0.032). The minor T allele of rs174537G>T was associated with lower risk of CAD [OR 0.77 (95% CI 0.63-0.95), P=0.015] after adjustment. The rs174537T allele carriers had significantly higher proportion of linoleic acid (LA, 18:2ω6) and lower arachidonic acid (AA, 20:4ω6) and the lower ratios of AA/dihomo-γ-linolenic acid (DGLA, 20:3ω6) and AA/LA than the G/G subjects in both control and CAD groups. In the control group, 174537T carriers had significantly lower levels of total- and LDL-cholesterol, malondialdehyde (MDA) and ox-LDL. In CAD patients, rs174537T carriers showed a larger LDL particle size than the G/G subjects. AA proportion in serum phospholipids positively correlated with LDL-cholesterol, ox-LDL and MDA in control subjects. Furthermore, AA positively correlated with 8-epi-prostaglandin F2α in both control and CAD groups. Conclusion: the rs174537 minor T allele is associated with lower proportion of AA in serum phospholipids and the reduced CAD risk. This is consistent with the observed association of the rs174537T allele with the reduced total- and LDL-cholesterol, and lipid peroxides.