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취효소 분비에 대한 부신피질홀몬의 영향

Authors
 윤태연 
Issue Date
1986
Description
의학과/박사
Abstract
[영문]

[한글]

부신피질홀몬은 체내 항상성 유지에 중요한 역할을 할 뿐아니라 임상적으로 각종 질환

치료에 효과적으로 쓰이고 있다. 부신피질홀몬은 동물 및 인체 취장의 세포분화, 효소생

성, 증식 및 비대에 관여한다고 보고되고 있다. 그러나 취효소 분비에 대하여는 부신피질

홀몬이 분비를 항진시킨다는 보고와 억제시킨다는 주장등 의견이 상반되고 있다. 대부분

천연산 및 합성 corticosteroid가 정도의 차이는 있으나 glucocorticoid작용과 mineraloc

orticoid작용을 모두 갖고 있어 실험결과의 해석에 무리가 있다.

따라서 이번 실험에서는 glucocorticoid작용만 가진 dexamethasone과 mineralocorticoi

d작용만 가진 desoxycorticosterone acetate(DOCA)를 선택하여 정상 또는 부신절제 흰쥐

의 취효소분비에 대한 이들 약물의 영향을 검토하고 또 생체외표본에서 부신피질홀몬의

직접 작용을 관찰하고자 하였다.

생체내 실험으로 몸무게 150g 안팎의 흰쥐를 사용하여 정상 동물군과 부신절제군으로

나누었으며 corticosteroids를 각각 매일 0.6mg/kg씩 1주간 주사한 후 urethane으로 마취

하여 담취액을 채취하였다. 생체외 실험으로는 60∼80g의 어린 흰쥐의 uncinate부분을 적

출하여 KRBB 용액내에서 corticosteroids처치에 따른 amylase유리 변동을 측정하였다. 또

한 생체의 실험으로 고양이 적출 취장관류 실험도 병행하였다.

이번 실험에서 얻은 결과는 다음과 같다.

1. 정상 흰쥐의 담취액 분비량은 0.19±0.01ml/100g/hr, amylase분비는 99.2±8.5IU/10

0g/hr이었으며, dexamethasone(0.6mg/kg/day, i.m.) 1주간 투여로 amylase분비는 감소되

었으나 DOCA(0.6mg/kg/day, i.m.) 투여로는 별 영향이 없었다.

2. 부신 절제로 흰쥐의 담취액 및 amylase분비량은 현저히 증가되었으나 dexamethasone

투여로 amylase분비는 억제되어 정상 흰쥐에서의 분비와 비슷하였다.

3. 정상 흰쥐에서 채취한 취절편의 amylase기초유리는 DOCA 10**-6 M첨가로 의의있게

증가하였으나 acetylcholine반응은 변동이 없었다.

4. 부신 절제후 1주에 채취한 취절편의 amylase기초유리는 dexamethasone 10**-6 M첨가

로 억제되었으며 acetylcholine반응은 DOCA첨가로 항진되고 dexamethasone첨가로 억제되

었다.

5. 고양이 적출 관류취장에서 dexamethasone은 취액분비와 CCK자극 amylase분비를 감소

시키는 경향을 나타내었다.

이상의 결과로 보아 부신피질홀몬은 취장기능 조절에 중요한 역찰을 하며 glucocortico

id는 억제적으로 작용하나 mineralocorticoid작용과의 군형에 의하여 그 영향은 크게 달

라질 수 있다고 생각한다.





Effect of Corticosteroids on the Secretion of Pancreatic Enzymes



Tae Yun Youn

Department of Medical Science The Graduate School, Yonsei University

(Directed by Professors Sa Suk Hong, M.D. and Choon Kyu Kim, M.D.)



Corticosteroids are important regulators of homeostasis and they are used widely

as effective therapeutic agents in clinical medicine. It has been claimed that

adrenal cortex and corticosteroids play an important role in maintaining the

trophism and the exocrine functions of the pancreas in experimental animal as well

as in human. However, the precise effects of corticosteroids exerted on pancreatic

exocrine function are still contradictory. Since most of corticosteroids have both

glucocorticoid and mineralocorticoid activities, the interpretations of the results

are sometimes intriguing. Present experiment was undertaken to clarify the role

played by corticosteroids on the enzyme secretion of exocrine pancreas employing

both in vivo as well as in vitro preparations. Pure glucocorticoid, dexamethasone,

and pure mineralocorticoid, desoxycorticosterone acetate(DOCA), were used.

The albino rats, normal and adrenalectomized, were treated with corticosteroids

(0.6 mg/kg, i.m.) for 7 days prior to starting the in vivo experiments and the

pancreatico-biliary juice was collected for 2-hours under urethane(1.25 g/kg, s.c.)

anesthesia.

For the in vitro experiments, baby rats, weighing 60∼80 g, were sacrificed and

the uncinate part of the pancreas was sliced. These pancreatic slices were

incubated in Krebs Ringer bicarbonate buffer(KRBB), with or without the presence of

steroids (10**-6 M) and the stimulation with acetylcholine(10**-6M). Also the

effects of steroids on the isolated saline perfused cat pancreas were examined.

The results are summarized as follows.

1. The volume of the pancreatico-biliary secretion from normal rats was

0.19±0.01 m1/100 g/hr, and the amylase secretion was 99.2±8.5 IU/100 g/hr.

Dexamethasone treatment suppressed the amylase secretion.

2. Adrenalectomy has caused marked increase of amylase secretion but

dexamethasone treatment for 1 week has decreases the amylase secretion to the level

of normal rats.

3 The amount of amylase released from the pancreatic slices of the normal rat was

significantly increased by the addition of DOCA to the incubation medium but the

acetylcholine-stimulated release of amylase from the pancreatic slices was not

influenced by either corticosteroids.

4. The ability to release amylase from the pancreatic slices of the

adrenalectomized rat was significantly inhibited by the addition of dexamethasone

to the incubation medium and the acetylcholine-stimulated release was augmented by

DOCA and decreased by dexamethasone.

5. In the isolated perfused cat pancreas, the pancreatic flow and the

CCK-stimulated secretion of amylase, tended to be decreased by dexamethasone.

By these findings it is suggested that the corticosteroids exert significant

influence on the exocrine secretory function of the pancreas, and the

glucocorticoid inhibits the secretory function. However, this inhibitory effect of

glucocorticoid might be greatly influenced by its balance with intrinsic

mineralocorticoid action.
Full Text
https://ymlib.yonsei.ac.kr/catalog/search/book-detail/?cid=CAT000000045422
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Appears in Collections:
1. College of Medicine (의과대학) > Others (기타) > 3. Dissertation
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/135732
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