근육활동에 의한 말초신경의 형태학적 연구

Abstract

[영문]

[한글]

운동이 말초신경에 미치는 영향을 보기 위하여 생후 30힐된 어린쥐와 성숙쥐를 두달 동

안 아침저녁으로 1시간씩 하루 2시간 수영을 시켰다. 제 4요신경의 전근과 후근을 절제하

여 소정방법에 따라 고정 및 탈수한 후 Epon 812에 포매하여 1㎛두께로 잘라 광학현미경

으로 관찰하고 700배로 한 시야씩 건너뛰며 사진을 찍은 다음 최종배율이 2,000배가 되도

록 인화하였다.

직경의 계측은 원형은 1회, 타원형은 장경과 단경을 각각 계측하여 평균값을 이용하였

고 수초의 두께는 얇은 쪽을 택해 1회 계측하였다.

좌골신경섬유는 10∼30배 입체현미경 하에서 분리하고 결절간거리는 100배에서, 직경은

450배에서 ocular micrometer로 계측하였다.

모든 계측치는 SPSS package program을 이용하여 컴퓨터로 처리하였다.

전근은 어린쥐의 경우 직경과 수초두께가 운동군에서 증가하였으며 성숙쥐에서는 변화

가 없었다.

후근은 직경과 수초두께가 어린쥐와 성숙쥐 모두의 운동군에서 감소를 보였다.

좌골신경의 직경과 결절간거리는 대조군에 비해 운동군에서 보다 더 직선적인 상관관계

를 보였다.

Quantitative Histological Studies of the Peripheral Nerve Influenced by Muscular

Activity of the Rats

Byoung Kee Kim

Department of Medical Science The Graduate School, Yontei University

(Directed by Prof. Tai Sun Shin, M.D. and In Hyuk Chung, M.D.)

In order to observe the effects of physical exercise on the peripheral nerve, 30

day old young rats and adult rats were exercised br swimming day and night, one

hour each for 2 months.

A portion of the anterior and posterior roota of each 4th lumbar nerve was

removed, fixed, dehydrated as usual, embedded in Epon 812 and then sectioned 1

micron thick. The setions were observed under the 700× magnification, and

photographed while scanning the different fields. The prints were finally enlarged

to 2000× magnification.

The diameter for the circularly sectioned fibers, and the average diameter of the

major and minor for the ovoidally sectioned filers were measured. The thickness of

the thinner part of the myelin sheath was measured.

The sclatic nerve fiber was isolated under stereoscopic microscope at 10∼30×

magnification. The internodal length and the diameter were measures 100× and 450×

respectively by the uke of an ocular micrometer.

The SPSS package computer program was used for analysis of the data.

The diameter of the anterior root and the thickness of myelin sheath were

increased in the exercised young rats, whereas no change was observed in the adult

rata.

The diameters of the posterior root and the thickness of the myelin sheath of

both young and adult rata were decreased in the exercised group.

The exercised group showed a more linear relationship between the internodal

length and the axonal diameter of the sciatic nerve than that of the control group.