合成卵巢 steroid가 초파리 遺傳物質에 미치는 영향

Abstract

[영문]

[한글]

The Genetic Effect of A Synthetic Ovarian Steroid in Drosophila melanogaster

Won Chung Kim, B.S.

Department of Public Health, Graduate School, Yonsei University

(Directed by Assistant Professor Jong Sik Yoon, Ph.D.)

For the purpose of evaluating the genetic effect of a synthetic ovearian steroid

in Drosophila melanogaster, Lyndiol 2.5 was used as a chemical mutagen. One to

three day old larvae of the sc**8·Y·B**s /y**2 W**i ct**6 f**1 flies were fed on

the medium containing 0.5ml of Lyndiol (50% in Drosophila Ringer's) solution for

treatment. Germ cells of male flies treated on the medium throughout larval, pupal

and imaginal stages of development were tested for mutations through two

generations.

When males treated were crossed individually to multipurpose virgin (y sc**61

In49sc**8 : dp Bw: st p**p), increased losses of the Y chromosome, nondisjunctions

and other visible mutations were found. Among the 12 visible mutants, 6 mosaics in

wing and eye were observed, suggesting that somatic mutations occurred

concomitantly with the germinal havoc detected.

An increased rate of sex-linked recessive lethal mutation (0.49%) was found, when

the visibly unaffected F^^1 inseminated females were tested by the Muller-t method.

This would be estimated some 2.5% recessive lethal mutations in the whole set of

chromosomes of treated males.

No translocation was found by crossing the visibly unaffected males to

multipurpose virgin females individually.

From the data accounted, it is possible to conclude that there have to be

mutagenic effect by the synthetic ovarian steroid in D.melanogaster.