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Alveolar bone resorption induced by CD4+CD45RB high-density T-cell transfer in immunocompromised mice
DC Field | Value | Language |
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dc.contributor.author | 김보경 | - |
dc.date.accessioned | 2015-12-24T09:05:32Z | - |
dc.date.available | 2015-12-24T09:05:32Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/134938 | - |
dc.description | Dept. of Dental Science/박사 | - |
dc.description.abstract | Objectives: The aims of this study were to determine whether the antigen-inexperienced [naive, CD45RB high density] T-cell (CD4+CD45RBHigh T-cell) transfer model is associated with alveolar bone resorption, to elucidate the local osteogenic/adipogenic potential of alveolar bone marrow stromal cells (ABCs) from T-cell-transferred animals, and to investigate the systemic osteogenic potential by transplanting human periodontal ligament stem cells (hPDLSCs) into these animals.Materials and Methods: CD4+CD45RBHigh and CD4+CD45RBLow [antigen-experienced (memory, CD45RB low density)] T cells were sorted and transferred into severe combined immunodeficiency (SCID) mice to induce inflammatory-bowel-disease (IBD)-like syndrome (n = 8). hPDLSCs were transplanted into T-cell-transferred SCID mice to examine ectopic cementum formation 8 weeks after T cell transfer. The mandibles and tibias of these mice were retrieved for micro-computed tomography (micro-CT), histomorphometric analysis, and isolation of alveolar bone cells 16 weeks after T cell transfer. The in vitro osteogenic and adipogenic potentials of the ABCs were evaluated.Results: Histological and micro-CT analysis revealed that the transfer ofCD4+CD45RBHigh T-cell subset was sufficient for alveolar bone resorption and affected the osteogenic/adipogenic potential of ABCs. Furthermore, it was found that CD4+CD45RBHigh T cell-transferred animals have decreased systemic osteogenic potential, as evidenced using the in vivoectopic hPDLSC transplantation model.Conclusion: CD4+CD45RBHigh T-cell transfer induced both alveolar bone resorption and reduced systemic osteogenic potential, with a concomitant down regulation of the osteogenic potential of ABCs. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Alveolar bone resorption induced by CD4+CD45RB high-density T-cell transfer in immunocompromised mice | - |
dc.title.alternative | CD4+CD45RBhigh T 면역세포를 처치한 면역억제 마우스에서 치조골 흡수 양상에 관한 연구 | - |
dc.type | Thesis | - |
dc.contributor.alternativeName | Kim, Bo Kyoung | - |
dc.type.local | Dissertation | - |
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