Inhibition of artificial caries around bioactive glass-ursolic acid containing composite resin

Abstract

1. ObjectiveRecurrent caries formation around existing restorations represents the primary reason for replacement of composite resin restorations. Use of the antimicrobial restorative material to inhibit the bacterial biofilm formation can be one way to prevent the recurrent caries around restoration. The aim of this study is to assess the inhibitory effect of experimental composite resins containing BAG and/or UA on artificial caries model.2. Materials and methodsFour antibacterial composites containing bioactive glass (BAG) filler, BAG filler coated with ursolic acid (UA BAG), ursolic acid was added to the resin matrix (BAG + UA Monomer), ursolic acid was added both BAG filler and resin matrix (UA BAG + UA Monomer) were developed. Conventional composite (OX50) were used as control groups. Standard cavities (3.5 mm × 2 mm × 2 mm) were prepared on the buccal and lingual surfaces of fifty extracted human third molars and restored with these composite resins (n=10). Artificial caries lesions were produced on the experimental teeth using S. mutans-induced artificial caries system. Demineralized area of dental enamel around the restorations were analyzed with quantitative light-induced fluorescence (QLF). The demineralized specimens were photographed with QLF-D BiluminatorTM 2 (Inspektor Research Systems bv, Amsterdam, Netherlands) and the fluorescence images of white spot area 1mm around the restorations were analyzed using image analysis software QA2 (Inspektor Research Systems bv, Amsterdam, Netherlands).3. ResultMean fluorescence loss (∆F) and the total fluorescence loss (∆Q) of experimental groups significantly decreased than control group, but there was no significant difference among experimental groups.4. ConclusionThe experimental composite resins containing bioactive glass and/or ursolic acid can inhibit the artificial caries induced by S. mutans biofilm around the restoration