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Serum Levels of Anti-Type VII Collagen Antibodies Detected by Enzyme-Linked Immunosorbent Assay in Patients with Epidermolysis Bullosa Acquisita are Correlated with the Severity of Skin Lesions

Other Titles
 후천성 수포 박리증 환자에서 type VII collagen의 NC1 과 NC2 recombinant protein을 이용한 ELISA 수치와 임상적 중증도의 비교 
Authors
 김종훈 
Issue Date
2012
Description
의학과/석사
Abstract
Epidermolysis bullosa acquisita (EBA) is a chronic autoimmune subepidermal bullous disease characterized by circulating autoantibodies against type VII collagen. Detecting these autoantibodies is crucial for the diagnosis of this disease, and is also useful for measuring disease severity. Enzyme linked immunosorbent assay (ELISA), a quantitative method to measure anti-type VII collagen antibody levels, is currently available to diagnose EBA. The aim of this study was to validate the usefulness of type VII collagen ELISA in diagnosing EBA and monitoring disease severity. Sera from patients with EBA (n=30), bullous pemphigoid (n=20), anti-laminin γ1 pemphigoid (n=9), and healthy donors (n=24) were tested by ELISA using the recombinant noncollagenous 1 (NC1) and 2 (NC2) domains of type VII collagen. Relationships between clinical characteristics, indirect immunofluoroscence (IIF) titers and ELISA values were investigated. The sensitivity and specificity of the type VII collagen ELISA were 96.7% and 98.1%, respectively. There was no significant difference between ELISA results for classic and inflammatory types (72.6 vs. 47.4 U/ml, p=0.15). The severity of skin involvement was positively correlated with both ELISA index value (r=0.87, p<0.01) and IIF titer (r=0.59, p<0.01). Time sequence analysis in 4 patients with EBA showed that ELISA index values reflect disease severity better than IIF titers. Type VII collagen ELISA using the NC1 and NC2 domains is useful for diagnosing EBA and monitoring disease severity.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/134209
Appears in Collections:
2. Thesis / Dissertation (학위논문) > 1. College of Medicine (의과대학) > Master's Degree (석사)
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