The bone regenerative effects of Chinese hamster ovary cell-expressed recombinant human bone morphogenetic protein-2 using demineralized bone matrix carrier in rabbit cranial defect

Abstract

Recombinant human bone morphogenetic protein-2 (rhBMP-2) has long been believed to have the potential to accelerate the healing process and augment tissue formation in challenging regenerative procedures. The objective of this study was to evaluate the bone regenerative effects of DBM coated by newly-developed Chinese hamster ovary (CHO) cell-expressed rhBMP-2 in rabbit cranial defect.Two symmetric, 11-mm ø, critical-size osteotomy defects were created in 7 New Zealand white rabbit craniums received DBM and rhBMP-2 coated DBM, respectively. Implant sites were evaluated by microcomputed tomography and histologic and histometric parameters following an 8-week healing interval. Surgical implantation of rhBMP-2 coated DBM resulted in enhanced local bone formation at 8 weeks. In defect closure, the rhBMP-2 coated DBM group was statistically significant greater than the DBM group (P<0.05). Total augmented area was not significantly different between the DBM group and the rhBMP-2 coated DBM group. However, New bone area of the rhBMP-2 coated DBM group was a significantly greater than those of the DBM group (P<0.05).In conclusion, DBM coated by newly-developed CHO-rhBMP-2 produced significant new bone and tissue formation in rabbit cranial defect. It is suggested that DBM may be an available carrier for rhBMP-2.