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Osteoinductive activity of biphasic calcium phosphate with different rhBMP-2 doses in rats
DC Field | Value | Language |
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dc.contributor.author | 장지웅 | - |
dc.date.accessioned | 2015-12-24T08:43:47Z | - |
dc.date.available | 2015-12-24T08:43:47Z | - |
dc.date.issued | 2011 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/134088 | - |
dc.description | Dept. of Dentistry/석사 | - |
dc.description.abstract | Recombinant human bone morphogenetic protein (rhBMP)-loaded biphasic calcium phosphate (BCP) ceramics, which comprise hydroxyapatite (HA)/beta-tricalcium phosphate (-TCP), can greatly accelerate bone formation. However, few studies have investigated the osteoinductive activity of BMP-loaded BCP with higher ratios of -TCP to HA. The aims of the current study were threefold: (i) to determine whether a HA/-TCP ratio of 20/80 (BCP 20/80) impregnated with rhBMP-2 enhances new bone formation in rat calvarial defects, (ii) to determine the dose-dependent response of rhBMP-2, and (iii) to elucidate the relationship between the amount of newly formed bone and graft degradation in BCP 20/80 at healing intervals of 2 and 8 weeks.Critical-sized calvarial defects were made in rats, which were then allocated to one of the following six groups: (1) sham-surgery control, (2) BCP control, (3) 2.5-μg rhBMP-2/BCP, (4) 5-μg rhBMP-2/BCP, (5) 10-μg rhBMP-2/BCP, or (6) 20-μg rhBMP-2/BCP. The animals were allowed to heal for either 2 or 8 weeks, after which they were sacrificed for histologic and histometric analyses.Histometric analysis showed that the percentages of new bone after 2 and 8 weeks of healing were significantly greater in the BMP-2-treated groups (at all doses) than in the control groups. The percentage of remaining BCP was significantly lower at 8 weeks than at 2 weeks in both the rhBMP-2-treated groups and in the BCP control group, but the amount of new bone was not significantly greater at 8 weeks than at 2 weeks in the 10-μg and 20-μg rhBMP-2/BCP groups.rhBMP-2 administered using a BCP carrier significantly induces new bone formation in the rat calvarial defect model. A dose-dependent response was not shown in the present study, and there appears to be no meaningful relationship between the amount of new bone formation and graft degradation with BCP 20/80. BCP 20/80 may be considered an effective and biocompatible a carrier for rhBMP-2. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Osteoinductive activity of biphasic calcium phosphate with different rhBMP-2 doses in rats | - |
dc.title.alternative | 백서 두개골 결손부에서 서로 다른 농도의 rhBMP-2가 적용된 이상(biphasic) 칼슘 포스페이트의 골유도 효과 | - |
dc.type | Thesis | - |
dc.contributor.alternativeName | Jang, Ji Woong | - |
dc.type.local | Thesis | - |
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