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Chitinase-like protein YKL-40 regulates hyperoxia-induced apoptosis in human airway epithelial cells

DC Field Value Language
dc.contributor.author김미나-
dc.date.accessioned2015-12-24T08:33:47Z-
dc.date.available2015-12-24T08:33:47Z-
dc.date.issued2012-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/133695-
dc.descriptionDept. of Medical Science/석사-
dc.description.abstractYKL-40 is chitinase-like protein that lacks chitinase activity. Prolonged exposure to 100% oxygen causes hyperoxic acute lung injury characterized by alveolar epithelial cell injury and death. We investigated the role of YKL-40 regulating hyperoxia-induced apoptosis in human airway epithelial cells. Human airway epithelial cell line, BEAS-2B, was exposed to > 93% oxygen for 24-72 hours. Hyperoxia induced apoptosis was confirmed by flow cytometry with Annexin-V and PI staining. The mRNA and protein expression of YKL-40, caspase 3 and caspase 7 was determined by real time PCR and Western blotting. YKL-40 short hairpin RNA (shRNA) and over-expression vectors were transfected to cells to examine the requirement of YKL-40. Hyperoxia increased FITC-Annexin V positive cells compared with room air. Caspase 3 and 7 , representative apoptosis regulators, were increased by hyperoxia on mRNA and protein level. YKL-40 expression was also increased. YKL-40 shRNA transfected cells expressed lower level of caspase 3 and 7 mRNA and protein than untransfected cells after exposing to hyperoxia, whereas YKL-40 over-expression vector transfected cells showed higher level of caspase 3 and 7 than untransfected cells. We concluded that hyperoxia induced apoptosis associates with YKL-40 expression and that YKL-40 could be considered as one of apoptosis regulators in oxidative stressed human airway epithelial cells.-
dc.description.statementOfResponsibilityopen-
dc.publisherGraduate School, Yonsei University-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleChitinase-like protein YKL-40 regulates hyperoxia-induced apoptosis in human airway epithelial cells-
dc.title.alternative고농도 산소에 의한 인체 기도상피세포 손상에서 YKL-40 의 apoptosis 조절-
dc.typeThesis-
dc.contributor.alternativeNameKim, Mi Na-
dc.type.localThesis-
Appears in Collections:
1. College of Medicine (의과대학) > Others (기타) > 2. Thesis

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