마우스에서 성숙에 따른 복강거식세포 기능 및 지연형 과민반응 유발부위의 세균감염에 대한 저항력에 관하여

Abstract

[한글]

신생아와 성인간에 미생물감염에 대한 저항력의 차이를 설명하는 기전의 하나로 탐식세포의 기능차이에 기인함을 보고하고 있으며 그 근거로 탐식세포의 대사활성, 화학주성,살균능력 또는 opsonin활성의 차이 등을 보고하고 있으나 아직 그 기전이 확실히 규명되지는 않았다.

이에 저자는 생후 3주된 마우스 및 16주된 마우스에서 L. monocytogenes에 대한 감수성을 비교하고, 복강거식세포의 기능 및 지연형 과민반응과, 지연형 과민반응 유발부위의세균감염에 대한 저항력을 관찰하여 다음과 같은 결과를 얻었다.

1. 생후 3주된 마우스에서 L. monocytogenes의 LD^^50 는 마우스 체중 그람당 정맥내접종에서 5x10**3 , 복강내 접종에서 5x10**5 였으며, 16주된 마우스에서 LD**50 는 정맥내 접종에서 8x10**4 , 복강내 접종에서 5x10**6 로 3주된 마우스군에서 listeria감염에

대한 감수성이 높았다.

2. 복강추출세포 총수는 생후 3주된 군에서는 마우스당 (2.9±1.3)x10**6 , 생후 16주된 군에서는 마우스당 (10.0±5.2)x10**6 으로 생후 16주된 마우스에서 많았으나, 마우스

체중 그람당 복강 추출세포수에는 차이가 없었다.

3. 복강거식세포의 균탐식능은 마혈청함유배지 및 동종혈청함유배지의 두 경우 모두 생후 16주된 마우스군에서 높았으며, 균소화능은 두군간 차이가 없었다. 한편 마우스 연령

에 따른 혈청내 opsonin활성이 균탐식능 및 소화능에 미치는 영향은 관찰할 수 없었다.

4. 감작면양적혈구에 의한 복강거식세포의 EA rosette형성율을 관찰한 결과 생후 3주된 군에서는 11.9±0.9%, 16주된 군에서는 22.2±2.6%로 3주된 군에서 EA rosette형성율이저하되어 있었다.

5. L. monocytogenes 및 BCG에 대한 지연형 과민반응 유발 및 지연형 과민반응 유발 족저부에서 L. monocytogenes감염에 대한 국소적 저항력을 관찰한 바 생후 3주 및 16주된마우스 두 군에서 모두 지연형 과민반응 유발 및 국소적 균증식억제를 나타내었으며, 두군간 차이는 관찰할 수 없었다.

이상의 결과를 종합하여 보면 생후 3주된 마우스 및 생후 16주된 마우스에서 지연형 과민반응에 의한 세포성 면역 발현에는 차이가 없으나 생후 3주 마우스군에서는 복강거식세포의 탐식능 및 EA rosette형성율이 저하되어 있음을 관찰할 수 있다. 따라서 생후 3주된

마우스군의 L.monocytogenes감염에 대한 저항력의 저하기전의 일환으로 세포성 면역 발현의 차이보다는 거식세포의 기능저하가 관여할 것으로 추측한다.

Functions of Peritoneal Macrophages and Antibacterial Resistance at the Site of

Delayed Footpad Reaction in 3 week-old and 16 week-old Mice

Kyung Won Song

Department of Medical Science The Graduate School, Yonsei University

(Directed by Professor Joo Deuk Klm, M.D.)

Age related changes in susceptibility of vertebrates to infection, i.e., group B

beta-hemolytic streptococcus in human newborn (Eickhoff et al, 1964), murine

coronavirus(Pickel, 1981) and murine blastomycosis(Brass and Stevens, 1982) are

well documented.

It has been implicated that immaturity in inflammatory responses in neonates

would be the major determinant for the neonate infections. The functional

differences of phagocytes from neonates and adults, which is one of the most

important cell in inflammatory response have been studied in various aspects which

include chemotaxis(Kretschmer et al, 1976: Klein et al, 1977), response to

opsonins(Forman and Stiehm, 1969; McCraken and Eichenwald, 1971) microbicidal

activity(Kretschmer et al, 1976; Zeligs et al, 1977 : Orlowaki et al, 1976; Becker

et al, 1981 ;), and metabolic activities(Nerukar et al, 1977).

In this paper, the resistance to the L. monocytogenes infection, the functions of

peritoneal macrophages, delayed type hypersensitivity responses and the

antibacterial resistance at the site of delayed type hypersensitivity in 3 week-old

and 16 week-old mice were studied.

1. LD^^50 /g(mouse body weight) of L. monocytogenes in 3 week-old group were

5×10**3 (Ⅳ) and 5×10**5 (IP), compared to 8×10**4 (Ⅳ) and 5×10**5 (IP) in 16

week-old mice group.

2. Average number of peritoneal exudates cells from mice were(2.9±1.3)×10**4 in

3 week-old mice group and (10.0±5.2)×10**5 in 16 week-old mice group. The

difference in the average number of peritoneal exudate cells per mouse wt. gram

were not significant.

3. The engulfment abilities of peritoneal macrophages from 3 week-old mice were

found to be lower than those of 16 week-old mice, but the digestion abilities were

nearby same in two groups. These findings were not varied by the use of heterosera

(horse) or allosera (mouse) supplemented in medium.

4. Percentage of EA rosette with sensitized RBC was lower by peritoneal

macrophages from 3 week-old mice(11.9±0.9%) than that by peritoneal macrophages

from 16 week-old mice (22.2±2.6%).

5. Delayed type hypersensitivity reaction and the antibacterial resistance at the

sits of delayed footpad reaction were successfully induced in both group of mice,

but the age-dependent difference in both groups were not observed.

Therefore, higher susceptibility of 3 week-old mice to L. monocytogenes infection

compared with that of 16 week-old mice were demonstrated. The susceptibility

difference were correlated with the functional differences of peritoneal

macrophages from the animals. Thus functional differences in young mice in terms of

phagocytosis and rosette formation might be one of the determinants for the higher

susceptibility.

Other findings, such as the differences in delayed type hrpersensitivity response

and antibacterial resistance at the site of delayed type hypersensitivity in both

groups were not significant.

[영문]

Age related changes in susceptibility of vertebrates to infection, i.e., group B beta-hemolytic streptococcus in human newborn (Eickhoff et al, 1964), murine coronavirus(Pickel, 1981) and murine blastomycosis(Brass and Stevens, 1982) are well documented.

It has been implicated that immaturity in inflammatory responses in neonates would be the major determinant for the neonate infections. The functional differences of phagocytes from neonates and adults, which is one of the most important cell in inflammatory response have been studied in various aspects which

include chemotaxis(Kretschmer et al, 1976: Klein et al, 1977), response to opsonins(Forman and Stiehm, 1969; McCraken and Eichenwald, 1971) microbicidal activity(Kretschmer et al, 1976; Zeligs et al, 1977 : Orlowaki et al, 1976; Becker et al, 1981 ;), and metabolic activities(Nerukar et al, 1977).

In this paper, the resistance to the L. monocytogenes infection, the functions of peritoneal macrophages, delayed type hypersensitivity responses and the antibacterial resistance at the site of delayed type hypersensitivity in 3 week-old and 16 week-old mice were studied.

1. LD^^50 /g(mouse body weight) of L. monocytogenes in 3 week-old group were 5×10**3 (Ⅳ) and 5×10**5 (IP), compared to 8×10**4 (Ⅳ) and 5×10**5 (IP) in 16 week-old mice group.

2. Average number of peritoneal exudates cells from mice were(2.9±1.3)×10**4 in 3 week-old mice group and (10.0±5.2)×10**5 in 16 week-old mice group. The difference in the average number of peritoneal exudate cells per mouse wt. gram

were not significant.

3. The engulfment abilities of peritoneal macrophages from 3 week-old mice were found to be lower than those of 16 week-old mice, but the digestion abilities were nearby same in two groups. These findings were not varied by the use of heterosera (horse) or allosera (mouse) supplemented in medium.

4. Percentage of EA rosette with sensitized RBC was lower by peritoneal macrophages from 3 week-old mice(11.9±0.9%) than that by peritoneal macrophages from 16 week-old mice (22.2±2.6%).

5. Delayed type hypersensitivity reaction and the antibacterial resistance at the sits of delayed footpad reaction were successfully induced in both group of mice, but the age-dependent difference in both groups were not observed.

Therefore, higher susceptibility of 3 week-old mice to L. monocytogenes infection compared with that of 16 week-old mice were demonstrated. The susceptibility difference were correlated with the functional differences of peritoneal macrophages from the animals. Thus functional differences in young mice in terms of phagocytosis and rosette formation might be one of the determinants for the higher susceptibility.

Other findings, such as the differences in delayed type hrpersensitivity response and antibacterial resistance at the site of delayed type hypersensitivity in both groups were not significant.