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사염화탄소(CCI4)의 단회투여가 경수절단된 흰쥐의 간세포에 미치는 영향에 관한 실험적 연구

Other Titles
 Experimental studies of cordotomized rat liver after single dose of carbon tetrachloride 
Authors
 신태선 
Issue Date
1970
Description
의학과/박사
Abstract
[한글]

[영문]

In attempting to ellucidate the mechanism of the action of CCl^^4 toxicity on the liver several theories have been proposed, one of which is that this poison directly attacks both mitochondria and endoplasmic reticulum of the liver cells. Conversely, substantial evidences have been accumulated Supporting the theory proposed by Brody (1979) that extensive Stimulation of the sympathetic nervous system resulting in anoxia of the liver cells is a significant factor in the pathogenesis of hepatic lesions following oral administration of CCl^^4. On the

basis of the observation that cervical spinal cordotomy protects a rat from the hepatotoxic effect of a single dose of CCl^^4, the author was attracted to the concept of Brody rather than that of a direct attack as the mechanism of the action of carbon tetrachloride. The present investigation was carried out with a view of correlating the damage in the organoids of the liver cells (following CCl^^4 administration) to the protection of the cervical cordotomy against CCl^^4 hepatotoxicity, at the cellular or ultrastructural level.

Ninty·six healthy male, albino rats (Sprague-Dawley strain), weighing about 200 gm, were used in this experiment, and they were divided into 4 groups: (1) the normal, control group was received a single oral dose of 1.25 ml/kg of olive oil mixed with an equal volume of saline. (2) the condo-tomized group was received the same dose of olive oil; (3) the CCl^^4·intoxication group was received a site oral dose of 1.25 ml/kg of CCl^^4, dissolved in an equal volume of olive oil, (4) the cordotomized and CCl^^4·intoxicated group was operated and administered as group (3). Cordotomy was performed at the level of the 7th cervical vertebra under light ether anesthesia. Carbon tetrachloride was given one hour after cordotomy. Small slices of the liver tissue were freshly obtained 24hours after the administration of carbon tetrachloride. For light microscopy, small pieces of the livers were fixed, and either embedded in paraffin blocks or cut with the freezing microtome. Paraffin sections were stained with hematoxylin-eosin, and frozen sections were treated by the enzyme histochemical methods as follows. Some unfixed, frozen sections were stained with oil red O. To demonstrate the activity of adenosine triphosphatase, the method presented by Wachstein and Meisel (1957) was used. The activity of alkaline phosphatase was demonstrated by the modified method of Barka and Anderson (1963) from the calcium-cobalt sulfide technique of Gomori The Eranko's modified method (1952) of Gomori's technique was used for the activity of acid phosphatase.F or electron microscopy, the smallest pieces of the tissue were fixed in 2 per cent osmium tetroxide solution buffered with sodium cacodylate containing 0.2 M sucrose. Following fixation, the tissues were embedded in Epon 812, cut with the ultramicrotome, stained with uranyl acetate, and examined under Hitaohi HU·IIE electron microscope.

In hematoxylin·eosin and oil red 0 stained preparations, the hepatic lesions induced with CCl^^4, e.g., the fatty change of hepatic cells and the sinusoidal congestion were observed, while they were abolished in the cordotomized and CCl^^4 intoxicated group. The activities of both adenosine triphosphatase and alkaline phosphatase were diminished in the bile canaliculi and hepatic cells of CCl^^4-intoxicattion group, but they were restored to a large extent by the 7 th cervical cordotomy. As talc ultrastructural changes of carbon tetrachloride intoxication, the enlargement of mitochondria, the decrease of mitochondrial cristae and granules, the decrease and enlargement of agglomeration of endoplasmic reticulum, the increase of vesicles in number, the scalloping and focal dilation of nuclear membrane, and the aggregation of chromatin and interchromatin granules were observed. However, they were prevented in some hepatic cells of the animals in the group of chordotomy cambined with CCl^^4-intoxication. From the above results, it can be concluded that the hepatotoxic effects of carbon tetrachloride is prevented or inhibited by the cervical cordotomy, and it can be suggested that the hepatotoxcity of CCl^^4 may be due to hypoxia in the hepatic cell through the stasis in talc hepatic blood circulation caused by intense sympathetic discharge.
Full Text
https://ymlib.yonsei.ac.kr/catalog/search/book-detail/?cid=CAT000000044961
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Others (기타) > 3. Dissertation
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/126948
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