성숙흰쥐에 투여한 Capsaicin이 신경조직에 미치는 영향

Abstract

[한글]

Capsaicin은 동물에 주사하였을 경우 그 화학적 작용으로 인해 일시적으로 통증을 유발시키나, 신생 흰쥐에 투여하였을 경우 통증을 전달하는 신경섬유에 대해 신경독성물질로 작용하여 통각전달을 차단시키기도 한다고 알려져 있다. 이와 같은 작용을 하는 capsaici

n을 성숙 흰쥐에 주사하였을 경우 신경조직에 어떠한 영향을 주는 가를 살펴보기 위하여 조직화학적, 면역조직화학적 방법 및 전자현미경을 이용하여 관찰하였다. 성숙 흰쥐에 capsaicin 50mg/kg을 피하주사한 후 24시간, 48시간, 1주일, 1개월이 경과한 후 중추신경계

통, 말초신경계통 및 장신경계통의 변화를 관찰하여 다음과 같은 결과를 얻었다. 성숙 동물에 투여한 capsaicin은 중추신경계통에도 영향을 미쳐, 척수 뒤뿔에서 calcitonin gene-related peptide(CGRP)와 substance P에 대한 면역반응성이 급격히 감소하였으며 주사 후 1주일에 가장 낮은 반응성을 보였다가 1개월이 경과한 후에는 점차 회복되는 양상을 보이기 시작하였다. 척수신경절 세포에서도 capsaicin 주사 후에 CGRP와 substance P를 함유한 세포의 숫자가 크게 감소하였으며 이러한 감소현상은 1주일에 가장 심하였다가 1개월이 경과한 후에는 점차 회복되는 양상을 보이기 시작하였다. 장신경계통에서의 CGRP와 substance P에 대한 면역반응성은 capsaicin 투여 후 1주일에는 가장 심한 감소현상을

보였다가 주사 후 1개월이 경과한 후에는 역시 회복되는 양상을 보였다. 또한 단위면적당 장신경세포의 수는 줄지 않았으나 크기는 줄어들어 주사 후 1개월이 경과한 후에도 세포의 크기가 회복되지 않았다. 전자현미경 소견에서 말초신경 중 capsaicin에 가장 민감

하게 반응하는 곳은 요관으로 capsaicin 투여 후 24시간에 심한 변화를 초래하였으며, 척추신경절 세포도 역시 주사 후 24시간에 변화를 나타내었다. 말초신경과 장신경들은 capsaicin 투여 후 1주일이 지나서야 뚜렷한 변화를 보였으며 주사 후 1개월이 경과한 후에는 점차 회복되는 양상을 보이기 시작하였다. Capsaicin을 성숙 동물에 투여하였을 때 신경계에 형태적인 변화를 초래하지 않는다는 종래의 개념과는 달리 본 실험의 결과들로 미루어 보아 신경세포와 신경섬유들에 심한 퇴행성 변화를 초래하였으며 신경전달물질에도 극

심한 영향을 주는 것으로 생각되었다. 이러한 변화는 주사 후 1주일이 경과한 후에 가장 심하게 나타났으며 1개월이 경과한 후에는 회복되기 시작하는 양상을 보였다.

Changes of nervous tissue in adult rots treated with capsaicin

Bong Ok Kim

Department of Medical Science The Graduate School, Yonsei University

(Directed by Professor Kyung Ah Park)

Capsaicin, the Pungent algesic substance of the red pepper is known to be a

neurotoxic substance, interrupting the pain conducting pathway. To investigate the

effects on the adult animals, histochemical, immunohistochemical and

electronmicroscopical observation have been done after capsaicin treatment.

Capsaicin 50 m9/kg was injected subcutaneously to the adult rats and after 24

hours, 48 hours,1 week and 1 month later, central nervous system, peripheral

nervous system and enteric nervous system was investigated.

The results obtained are as follows;

The immunoreactivity against substance P(SP) and calcitonin gene related

peptide(CGRP) in the dorsal horn of spinal cord decreased by capsaicin treatment in

adult rats and the effect was maximal after 1 week. It began to recover from 1

month after capsaicin treatment.

In spinal ganglia, the number of CGRP and SP containing neurons decreased and

these phenomenon was maximal in 1 week and began to recover from 1 month after

capsaicin treatment.

In enteric nervous system, the immunoreactivity against CGRP and SP was decreased

from 24 hours after capsaicin treatment and in 1 week, almost no reactivity was

observed. These were also recovered from 1 month after capsaicin injection. The

number of enteric neurons per unit area was not chanced but the sizes of the

neurons decreased and these decrement was not recovered until 1 month later.

In electronmicroscopical examination, the most sensitive part to capsaicin was

ureter and it showed severe morphological chanties after 24 hours. Dorsal root

ganglia cells also shewed changes after 24 hours. The other parts showed prominent

changes after 1 week and they showed recovery from 1 month after capsaicin

treatment.

On contrary to the past conception that adult capsaicin has no effect on animals,

capsaicin treatment on adult animals induced severe morphological changes to

neurons and nerve fibers and affect also to the neurotransmitters. These changes

were maximal in 1 week and began to recover from 1 month after capsaicin treatment.

[영문]

Capsaicin, the Pungent algesic substance of the red pepper is known to be a neurotoxic substance, interrupting the pain conducting pathway. To investigate the effects on the adult animals, histochemical, immunohistochemical and electronmicroscopical observation have been done after capsaicin treatment.

Capsaicin 50 m9/kg was injected subcutaneously to the adult rats and after 24 hours, 48 hours,1 week and 1 month later, central nervous system, peripheral nervous system and enteric nervous system was investigated.

The results obtained are as follows;

The immunoreactivity against substance P(SP) and calcitonin gene related peptide(CGRP) in the dorsal horn of spinal cord decreased by capsaicin treatment in adult rats and the effect was maximal after 1 week. It began to recover from 1 month after capsaicin treatment.

In spinal ganglia, the number of CGRP and SP containing neurons decreased and these phenomenon was maximal in 1 week and began to recover from 1 month after capsaicin treatment.

In enteric nervous system, the immunoreactivity against CGRP and SP was decreased from 24 hours after capsaicin treatment and in 1 week, almost no reactivity was observed. These were also recovered from 1 month after capsaicin injection. The number of enteric neurons per unit area was not chanced but the sizes of the neurons decreased and these decrement was not recovered until 1 month later.

In electronmicroscopical examination, the most sensitive part to capsaicin was ureter and it showed severe morphological chanties after 24 hours. Dorsal root ganglia cells also shewed changes after 24 hours. The other parts showed prominent changes after 1 week and they showed recovery from 1 month after capsaicin treatment.

On contrary to the past conception that adult capsaicin has no effect on animals, capsaicin treatment on adult animals induced severe morphological changes to neurons and nerve fibers and affect also to the neurotransmitters. These changes were maximal in 1 week and began to recover from 1 month after capsaicin treatment.