인삼이 흰쥐의 간미토콘드리아의 산화-인산화 과정에 미치는 영향

Abstract

[한글]

The Effect of Ginseng on the Oxidative Phosphorylation of Rat Liver Mitochondria in

Vitro

Sung Il Hong

Department of Medical Science The Graduate School Yonsei University

(Directed by Prof. Doo Hee Kang, M.D., Ph.D.)

Numerous studies on pharmacological actions of ginseng (Panax Ginseng C. A.

Meyer) on biological functions including carbohydrate metabolism (Petkov, 1958;

Kang et al., 1962; Cheong et al., 1965), energy metabolism (Lee et al, 1962) and

physical performance (Brekhman et al., 1969) have been reported. However the effect

of ginseng on the oxidative phosphorylation of mitochondria have not been reported.

This investigation was aimed to elucidate some mechanisms of the effect(s) of

ginseng on oxidative phosphorylation of rat liver mitochondria.

The liver mitochondria of rats were isolated by the method of Hogeboom (1955).

Oxygen consumption at state 4 and state 3 were measured polarographically using a

stationary platinum electrode. The amount of ATP synthetized after an addition of

ADP to incubation medium in the process of phosphorylation was determined

enzymatically with the dessicated tails of fire flies (Van Dyke et al., 1969). The

respiratory control ratio and P/O ratio was calculated from the above results.

Changes in optical density of mitochondrial suspension were measured with

spectrophotometer (at 520mu) in the presence of varying concentration of ginseng

ethanol extract or ginseng saponin.

The results were summarized as follows;

1. The rate of oxygen consumption of mitochondria at state 4 was increased as the

concentration of ginseng ethanol extract or saponin was increased. Accordingly, the

respiratory control ratios were decreased significantly.

2. Similarly, decreases in P/O ratio were found in the presence of ginseng

ethanol extract or ginseng saponin in the incubation mixtures.

3. Likewise, decrease in optical density of mitochondria suspension in the

presence of the saponin or the extract was also found.

4. Ginseng saponin was found ti be more potent in the decrease of P/O ratio and

respiratory control ratio. It is speculated that ginseng saponin also acts as a

detergent on the mitochondrial membrane.

[영문]

Numerous studies on pharmacological actions of ginseng (Panax Ginseng C. A. Meyer) on biological functions including carbohydrate metabolism (Petkov, 1958; Kang et al., 1962; Cheong et al., 1965), energy metabolism (Lee et al, 1962) and physical performance (Brekhman et al., 1969) have been reported. However the effect of ginseng on the oxidative phosphorylation of mitochondria have not been reported. This investigation was aimed to elucidate some mechanisms of the effect(s) of ginseng on oxidative phosphorylation of rat liver mitochondria.

The liver mitochondria of rats were isolated by the method of Hogeboom (1955). Oxygen consumption at state 4 and state 3 were measured polarographically using a stationary platinum electrode. The amount of ATP synthetized after an addition of ADP to incubation medium in the process of phosphorylation was determined enzymatically with the dessicated tails of fire flies (Van Dyke et al., 1969). The respiratory control ratio and P/O ratio was calculated from the above results.

Changes in optical density of mitochondrial suspension were measured with spectrophotometer (at 520mu) in the presence of varying concentration of ginseng ethanol extract or ginseng saponin.

The results were summarized as follows;

1. The rate of oxygen consumption of mitochondria at state 4 was increased as the concentration of ginseng ethanol extract or saponin was increased. Accordingly, the respiratory control ratios were decreased significantly.

2. Similarly, decreases in P/O ratio were found in the presence of ginseng ethanol extract or ginseng saponin in the incubation mixtures.

3. Likewise, decrease in optical density of mitochondria suspension in the presence of the saponin or the extract was also found.

4. Ginseng saponin was found ti be more potent in the decrease of P/O ratio and respiratory control ratio. It is speculated that ginseng saponin also acts as a detergent on the mitochondrial membrane.