Cited 0 times in

62 0

Acanthamoeba culbertsoni 감염에 있어 자연살세포의 활성에 관한 연구

Other Titles
 Natural killer cell activity in mice infected with acanthamoeba culbertsoni 
Issue Date
1991
Description
의학과/박사
Abstract
[한글] 원발성 아메바성 수막뇌염을 일으키는 병원성이 강한 자유 생활 아메바인 Acanthamoeba culbertsoni의 영양형을 C3H/HeJ 마우스에 1×10**4 과 1×10**5을 각각 감염시킬 후 12시간, 1일, 2일, 5일, 17일째에 회생하여 비장 세포의 YAC-1 표적세포에 대한 세포독성을 (51)**Cr 방출 검사법으로 측정하였고 단세포 독성 검사법으로 표적 세포 결합능, 활성 자연살세포, 더 나아가 최대 자연살세포 살해능 및 최대 재순환능을 측정하였다. 감염 마우스의 사망률은 아메바 영양형 1×10**4 을 감염시켰을 때 34%였으며 1×10**5 을 감염시켰을 때는 65%였다. 또한 접종 후 20일까지 사망한 마우스의 평균 생존 기간은 각각 16.40±3.50, 13.20±4.09 일 이었다. 자연살세포의 세포 독성은 두 실험군 모두에 서 아메바 접종 후 12시간째에 대조군에 비하여 유의하게 증가하기 시작하여 접종 후 1일째 가장 높았다. 2일 후에는 대조군과 비슷한 수준을 보였고 접종 후 10일째에는 대조군에 비해 저하되었으며, 두 실험군 간에 차이는 발견할 수 없었다. 아메바 영양형 1×10** 5 을 감염시킨 군에서 표적 세포 결합능은 접종 후 12시간, 1일째에 유의하게 증가하였고 활성 자연살세포도 비슷한 경향으로 증가하였으나 재순환능은 감염 경과 시간별로 대조군과 비교하여 차이를 발견할 수 없었다. 이상의 결과를 종합하면 Acanthamoeba culbertsoni 감염에 의해 비장세포의 자연살세포 독성이 증가하였는데 이는 자연살세포의 표적세포와의 결합능과 활성 자연살세포수의 증가와 유의한 상관관계가 있고 자연살세포의 재순환능과는 무관한 것으로 나타났다. Natural killer cell activity in mice infected with Acanthamoeba culbertsoni Dong-Kun Hynn Department of Medical Science, The Graduate School, Yonsei University Directed by Prof. Kyung-il Im, M.D. and Associated Prof. Han-Il Lee, Ph.D.) The natural killer cell activity of splenocytes and TBC, active NK cell, recycling capacity of natural killer cells were observed by means of both the (51)**Cr -release cytotoxicity assay (U1berg and Jondal, 1981) and sin히e cell cytotoxicity assay against YAC-1. The C3H/HeJ mice were infected intranasally with 1×10**4 and 1×10**5 Acanthamoeba culbertsoni trophozoites respectively that are Pathogenic free-living amoeba. The mice infected with 1×10**4 and 1×10**5 Acanthamoeba culbertsoni trophozoites showed mortality rate of 34% and 65% respectively, and mean survival time is 16.40±3.50 and 13.20±4.09 days respectively. The cytotoxic activity of natural killer cell of two experimental groups was significantly higher than that of non-infected mice during the period between the 12th hour the 2nd day and after infection, showing the highest on the first day. On the 17th day after infection the natural killer cell cytotoxic activity was significantly suppressed as compared with the control group. There was no significant difference between two infected groups. The target-binding capacity and active NK cells of natural killer Cells in 1×10**5 Acanthamoeba culbertsoni trophozoites infected mice was significantly increased on the 12th hour and the first day after infection as compared with the control group. Maximal recycling capacity (MRC) was not changed during the infected period. The results obtained in the experiments indicated that the elevation of natural killer cell activity in the mice infected with Acanthamoeba culbertsoni was due to the elevations of target-binding capacity and active NK cells of natural killer cell, and not due to the maximal recycling capacity of the individual NK cells, and there was no difference between two experimental groups.
[영문] The natural killer cell activity of splenocytes and TBC, active NK cell, recycling capacity of natural killer cells were observed by means of both the (51)**Cr -release cytotoxicity assay (U1berg and Jondal, 1981) and sin히e cell cytotoxicity assay against YAC-1. The C3H/HeJ mice were infected intranasally with 1×10**4 and 1×10**5 Acanthamoeba culbertsoni trophozoites respectively that are Pathogenic free-living amoeba. The mice infected with 1×10**4 and 1×10**5 Acanthamoeba culbertsoni trophozoites showed mortality rate of 34% and 65% respectively, and mean survival time is 16.40±3.50 and 13.20±4.09 days respectively. The cytotoxic activity of natural killer cell of two experimental groups was significantly higher than that of non-infected mice during the period between the 12th hour the 2nd day and after infection, showing the highest on the first day. On the 17th day after infection the natural killer cell cytotoxic activity was significantly suppressed as compared with the control group. There was no significant difference between two infected groups. The target-binding capacity and active NK cells of natural killer Cells in 1×10**5 Acanthamoeba culbertsoni trophozoites infected mice was significantly increased on the 12th hour and the first day after infection as compared with the control group. Maximal recycling capacity (MRC) was not changed during the infected period. The results obtained in the experiments indicated that the elevation of natural killer cell activity in the mice infected with Acanthamoeba culbertsoni was due to the elevations of target-binding capacity and active NK cells of natural killer cell, and not due to the maximal recycling capacity of the individual NK cells, and there was no difference between two experimental groups.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/117366
Appears in Collections:
2. 학위논문 > 1. College of Medicine (의과대학) > 박사
사서에게 알리기
  feedback
Fulltext
교내이용자 서비스로 제공됩니다.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse