Glutaraldehyde 및 Formocresol이 유견유치 치수조직에 미치는 영향에 관한 병리 조직학적 연구

Abstract

[한글]

저자는 생활치수절단술에 사용되는 약제들의 치수조직에 대한 반응을 비교하기 위하여 생후 약 8∼9주된 5마리의 유견에서 하악 유견치 및 유구치를 실험대상으로 하여, fomocresol, 2% glutaraldehyde, calcium hydroxide군으로 나누어 통법에 의한 생활치수절단술을 시행한 후 2시간, 1주, 2주, 3주, 5주군으로 각각 나누어 실험동물을 희생시키고, 표본을 10% formalin에 고정시킨후 5% nitric acid로 탈회하여 Hematoxylin-Eosin으로 염색한후 병리조직학적으로 관찰하여 다음과 같은 결론을 얻었다.

1. calcium hydroxide 실험군에서는 1주군에서 상아교의 형성이 시작되어 5주군에서 완성되었다.

2. formocresol, glutaraldehyde 실험군에서는 치수의 괴사가 나타났고 상아교의 형성은 없었다.

3. formocresol 실험군과 glutaraldehyde실험군에서는 서로 반응기간, 조직변화상등이 유사하였다.

4. formocresol, glutaraldelyde실험군에서 2시간, 1주군에서는 치수절단면이 혈병으로 피개되고, 그 직하부의 치수는 응고성 괴사를 일으키고, 2주군부터는 응고성 괴사가 치근단 방향으로 이행되고 염증세포의 침윤을 수반하며, 3주, 5주군에서는 화농과 액화성

괴사등이 보였다.

5. 화농된 치수는 유치 상아질의 흡수를 야기시키며 치근단 주위조직에 염증상이 나타나고, 치근의 흡수를 보였다.

6. calcium hydroxide실험군에 비해 glutaraldehyde와 formocresol 실험군에서 치근 흡수가 심하였고, 사용된 약제가 영구치배에 미치는 영향은 관찰되지 않았다.

A Histopathological Study of Pulp Tissue Reactions to Glutaralhehyde and

Formocresol in Puppy's Primary Teeth

No Jeong Hur, D.D.S., M.S.D.

Department of Dental Science, Graduate School Yonsei University

(Directed by Associate Prof. Jong Gap Lee, D.D.S., M.S.D., Ph.D.)

This study was undertaken to evaluate the pulpal responses to the pulp-capping

materials such as glutaraldehyde and formocresol in pulpotomy technique, especially

in the primary dentition.

Mandibular primary canines and molars of 5 dogs(aged about 8-9 weeks) were

selected for this study. The intervals of observation for histologic study of

pulpotomized primary teeth with 2% glutaraldehyde, formocresol and calcium

hydroxide in the usual manner ranged from 2 hours,1 week, 2 weeks, 3 weeks and 5

weeks after experiments respectively.

Each specimens were fixed with 10% formalin and decalcified in 5% nitric acid.

All slides were stained with Hematorylin-Eosin and examined histopathologically.

The results were as follows;

1. In calcium hydroxide groups, formation of dentin bridge was initiated in 1

week after experiments and completed in 5 weeks after experiments.

2. Formation of dentin bridge was not seen, whereas necrosis of pulp tissue was

noted in formocresol and glutaraldehyde groups.

3. Duration of tissue reactions and tissue changes were similar, in formocresol

and glutaralhehyde groups.

4. In formocresol and glutaraldehyde groups, amputation surfaces of the pulp were

covered with blood clots, beneath which coagulation necrois was noted, but

inflammatory cells ware not prominent in 2 hours and 1 week after experiments. But

coagulation necrosis was proceeded to the apical portion, accompanied by

infiltration of inflammatory cells, since 2 weeks after experiments. Suppuration or

gangrene of the pulp tissue were noted in 3 weeks and 5 weeks groups.

5. Suppuration or gangrene of pulp seemed to provoke the resorption of dentin

wall, and inflammatory changes and resorption of roots were noted in the

periodontal membrane near the periapical region.

6. As compared with calcium hydroxide groups, resorption of the root was

pronounced in formorcresol and glutaraldehyde groups. Effects of medicaments to the

succedaneous tooth germ were not seen.

[영문]

This study was undertaken to evaluate the pulpal responses to the pulp-capping materials such as glutaraldehyde and formocresol in pulpotomy technique, especially in the primary dentition.

Mandibular primary canines and molars of 5 dogs(aged about 8-9 weeks) were selected for this study. The intervals of observation for histologic study of pulpotomized primary teeth with 2% glutaraldehyde, formocresol and calcium hydroxide in the usual manner ranged from 2 hours,1 week, 2 weeks, 3 weeks and 5 weeks after experiments respectively.

Each specimens were fixed with 10% formalin and decalcified in 5% nitric acid.

All slides were stained with Hematorylin-Eosin and examined histopathologically.

The results were as follows;

1. In calcium hydroxide groups, formation of dentin bridge was initiated in 1 week after experiments and completed in 5 weeks after experiments.

2. Formation of dentin bridge was not seen, whereas necrosis of pulp tissue was noted in formocresol and glutaraldehyde groups.

3. Duration of tissue reactions and tissue changes were similar, in formocresol and glutaralhehyde groups.

4. In formocresol and glutaraldehyde groups, amputation surfaces of the pulp were covered with blood clots, beneath which coagulation necrois was noted, but inflammatory cells ware not prominent in 2 hours and 1 week after experiments. But coagulation necrosis was proceeded to the apical portion, accompanied by infiltration of inflammatory cells, since 2 weeks after experiments. Suppuration or gangrene of the pulp tissue were noted in 3 weeks and 5 weeks groups.

5. Suppuration or gangrene of pulp seemed to provoke the resorption of dentin wall, and inflammatory changes and resorption of roots were noted in the periodontal membrane near the periapical region.

6. As compared with calcium hydroxide groups, resorption of the root was pronounced in formorcresol and glutaraldehyde groups. Effects of medicaments to the succedaneous tooth germ were not seen.