이상의 연구결과 고환조직을 자가이식시 테스토스테론분비가 가능하였다. 그리고 조직량을 100mg(고환용적의 10%)내외로, 이식부위로는 신피막하부를 선택하는 것이 테스토스테론분비능이 가장 높았지만 혈중 테스토스테론농도가 정상수준의 25%에 지나지 않아 정
상수준의 회복을 위한 이식조건에 대한 연구는 계속되어겨야 할 것으로 생각된다.
[영문]
The present experiments were deviled to study the testosterone producing activity of the testicular autograft. Homogenate suspension of the testis containing interstitial cells were grafted in castrated Sprague-Dawley adult rats, Grafted rats were further divided into two experiments. To determine the minimum quantity of testicular tissue required for androgenic effect, graft masses were implanted in different volume(50mg, 100Hg and 250Hg). And to locate the optimum site for implantation, graft masses were implanted in various sites (subcutaneous tissue, intraperitoneum and renal subcapsular space). The results were assessed after 1 month by determining the serum testosterone and the weights of seminal vesicle and by the histological findings of the grafts and seminal vesicle.
Following transplantation of 100mg and 250mg of testicular tissue the serum testosterone and seminal vesicle weights were significantly higher than those of castrates and 5Omg grafted group(p< 0.05).
The serum testosterone and seminal vesicle weights of the groups given renal subcapsular and intraperitoneal grafts were significantly higher than those of castrated controls(p<0.05). The subcutaneous tissue was not effective site
Histological examination of the renal subcapsular grafts showed large collections of interstitial cells with atrophy of the seminiferous tubules.
From the above results testosterone producing activity of the testicular autograft was confirmed, and it is concluded that autotransplantation of 100mg of testicular tissue in the renal subcapsular space was most efficient. But the maximum testosterone level of the grafts was only approximately 25% of that of normal control in the present experiment, so the other method such as the transplantation of purified Leydig cells is needed to achieve complete normalization ofandrogenic effect.