취외분비선에 미치는 사염화탄소의 영향

Abstract

[한글]

약물은 주로 간장 smooth surfaced endoplasmic reticulum에 존재하는 효소에 의하여 대사되므로 약물의 작용기간이나 작용강도는 대략 이 효소의 대사속도에 따라 결정된다.

phenobarbital의 반복 투여는 약물대사 효소를 증가 시킬 뿐 아니라 외분비 기능도 증가시킨다. 담취외분비 기능에 있어서는 phenobarbital 반복투여로 담취액 분비량 및 bilirubin 배출을 증가시키며 amylase, lipase 활성을 현저히 증가시키나 cholate 농도는 감소시킨다고 보고되고 있다. 사염화탄소를 동물에 투여하면 간장 endoplasmic reticulum에 조직학적 변화를 일으키고 microsome 효소작용이 감소되며 간장 단백합성도 저하시킨다.

본 실험에 있어서는 이러한 약물대사효소 항진인자인 phenobarbital과 효소억제 물질인 CC/4를 투여하여 간 및 취외분비선에 미치는 영향을 비교 검토하였다.

본 실험에서 얻어진 결과를 요약해 보면 다음과 같다.

1. 사염화탄소 단독투여군의 치사율은 34%이나 phenobarbital 전처치후 사염화탄소 투여군에서는 치사율이 15%로 감소되었다.

2. phenobarbital 투여군에서는 담취액 분비량의 현저한 증가를 사염화탄소 투여군에서는 현저한 감소를 나타냈다.

3. 담취액 bilirubin 농도는 phenobarbital 단독 투여군에서는 감소를 사염화탄소 단독투여군에는 증가를 보였으며, phenobarbital 전처치후 사염화탄소 투여로 bilirubin 배출량과 농도가 현저히 증가되었다.

4. cholate 농도와 배출량은 대조군에 비해 모든 실험군에서 현저히 감소하였다.

5. 당취액 lipase 치는 phenobarbital 투여군과 phenobarbital 전처치후 사염화탄소 투여군에서 증가를 보였으나 사염화탄소 단독투여군에서는 별 변동을 볼 수 없었다.

6. 담취액 amylase치는 phenobarbital 단독투여군에서는 현저한 증가를, 사염화탄소 단독 투여군에서는 감소를 phenobarbital 전처치 후 사염화탄소 투여군에서는 경도의 증가를 보였다.

위의 사실들로 보아 사염화탄소 투여로 인한 간손상으로 간외분비 뿐 아니라 취외분비기능도 저하되나 phenobarbital 전처치로 사염화탄소의 간취독성이 강약 됨을 알 수 있다.

[영문]

The metabolism of many drugs and also of steroid hormones is mediated by enzymes located in the microsomal fraction in smooth surfaced endoplasmic reticulum of mammalian liver. The duration and intensity of action of many drugs are largely determined by the speed at which they are metabolized in the body.

Repeated administration of phenobarbital results in the induction of enzymes that metabolize a number of drugs. Lee et al. reported that daily administration of phenobarbital in rats significantly increased the activities of amylase and lipase in the pancreatobiliary juice, but the concentration of cholate in the bile was significantly lower in the treated group than that in the control group.

After animals were treated with CCl^^4, histological changes were shown in the endoplasmic reticulum, decreased microsomal enzyme activity and decreased hepatic protein synthesis were apparent.

The purpose of the present report was to study the interaction between a "microsomal-stimulating" agent such as phenobarbital and a "microsomal-depressing" agent such as CCl^^4 on hepatic and pancreatic functions in rats.

The results obtained are summarized as follows:

1. The mortality rate of Ccl^^4 treated group was 34% and was decreased this figure to 15% with phenobarbital pretreatment.

2. In animals treated with phenobarbital the volume of biliary-pancreatic secretion was markedly elevated but the volume was decreased significantly in animals treated with CCl^^4.

3. Total bilirubin output was elevated markedly in the CCl^^4. treated group of rats pretreated with phenobarbital. The bilirubin concentration was increased in CCl^^4 treated group and decreased in the group treated with phenobarbital alone.

4. The concentration and tal output of cholate in the bile were significantly lower in the all experimental group than control group.

5. In the animals treated with phenobarbital alone and phenobarbital plus CCl^^4, the activity of lipase in pancreatobiliary juice was elevated, while in the animals

treated with CCl^^4 alone no change was observed.

6. The activity of amylase in the pancreatobiliary juice was decreased in the CCl^^4 treated group, but elevated markedly in phenobarbital group and also elevated in phenobarbital CCl^^4 group.

By the above results, it is concluded when the liver was damaged by CCl^^4, the exocrine function of pancreas and liver was decreased simultaneously. However, in the animals pretreated with phenebarbital, the toxicity of CCl^^4 on the liver and

pancreas was reduced.