Cited 0 times in
성견악하선에서 Na+, K+ - activated Adenosine Triphosphatase에 대한 실험적 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김지학 | - |
dc.date.accessioned | 2015-11-20T04:51:01Z | - |
dc.date.available | 2015-11-20T04:51:01Z | - |
dc.date.issued | 1985 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/115567 | - |
dc.description | 치의학과/석사 | - |
dc.description.abstract | [한글]저자는 성견의 악하선에서 Na**+, K**+ -ATPase를 균등짙액, 과립체 분뵉, 블연속성 당 밀도등급도 및 연속성 당 밀도 등급도 순으로 순수 정제 분리를 시도하여 다음과 같은 성적을 얻었다. 1. 균등질액내 Na**+, K**+ -ATPase의 Specific activity는 0.63μmoles/min/mg·protein이었다. 2. 과립체 분획내 Ma**+, K**+ -ATPase의 specific activity는 2.79μmoles/min/mg·protein으로 균등질액에서 보다 4,4배 분리 정제되었으며 80% 효소 최수율을 나타내었다. 3. 불연속성 당 밀도 등급도내의 Na**+, K**+ -ATPase의 specific activity는 20.41μmoles/min/mg·protein으로 균등질액에서 보다 32.4배 분리 정제되었으며 20% 효소 회수율을 나타내었다. 4. 연속성 당 밀도 등급도내의 Na**+, K**+ -ATPase의 specific activity는 분획 11에서 가장 높게 나타났으며 , 그 때의 밀도는 .1.10-1.12g/ml이었고 specific activity는 36.29μmoles/min/mg·protein으로 균등질액에서 보다 57.6배 분리 정제되었다. [영문]Experimental study of purificating trials and specific activity of Na**+, K**+, -activity Adenosine Triphosphatase in dog submaxillary gland was done and results were obtained as follow ; 1. In homogenate, the specific activity of Na**+, K**+ -ATPase was 0.63μmoles/min/mg-protein. 2. In microsomal fraction, the specific activity of Na**+, K**+ -ATPase was 2.79μoles/min/mg·trotein. The enzyme was purified 4.4-fold than in homogenate and recovery rate was 80%. 3. In discontinuous gradient, the specific activity of Na**+. K**+ -ATPase was 20.41μmoles/min/mg·protein. The enzyme was purified 32.4-fold than in homogenate and recovery rate was 20%. 4. In linear gradient, the specific activity if Na**+, K**+-ATPase was the highest at fraction 11, and the density was 1.10∼1.12g/ml at that, The specific activity was 36.29μmoles/min/mg·protein, and the enzyme was purified 57.6-fold than in homogenate. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.publisher | 연세대학교 대학원 | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 성견악하선에서 Na+, K+ - activated Adenosine Triphosphatase에 대한 실험적 연구 | - |
dc.title.alternative | (An) experimental study on Na+, K+ - activated adenosine triphosphatase in dog submaxillary gland | - |
dc.type | Thesis | - |
dc.identifier.url | https://ymlib.yonsei.ac.kr/catalog/search/book-detail/?cid=CAT000000005744 | - |
dc.contributor.alternativeName | Kim, Jee Hack | - |
dc.type.local | Thesis | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.