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A Calcium Flux Is Required for Circadian Rhythm Generation in Mammalian Pacemaker Neurons

Authors
 Gabriella B. Lundkvist  ;  Yongho Kwak  ;  Gene D. Block  ;  Hajime Tei  ;  Erin K. Davis 
Citation
 JOURNAL OF NEUROSCIENCE, Vol.25(33) : 7682-7686, 2005 
Journal Title
JOURNAL OF NEUROSCIENCE
ISSN
 0270-6474 
Issue Date
2005
MeSH
Animals ; Calcium Signaling/physiology* ; Cell Cycle Proteins ; Cells, Cultured ; Circadian Rhythm/physiology* ; Mice ; Mice, Transgenic ; Neurons/physiology* ; Nuclear Proteins/physiology ; Period Circadian Proteins ; Rats ; Suprachiasmatic Nucleus/physiology*
Keywords
circadian rhythm ; calcium ; potassium ; suprachiasmatic nucleus ; Period 1 ; PERIOD 2
Abstract
Generation of mammalian circadian rhythms involves molecular transcriptional and translational feedback loops. It is not clear how membrane events interact with the intracellular molecular clock or whether membrane activities are involved in the actual generation of the circadian rhythm. We examined the role of membrane potential and calcium (Ca2+) influx in the expression of the circadian rhythm of the clock gene Period 1 (Per1) within the rat suprachiasmatic nucleus (SCN), the master pacemaker controlling circadian rhythmicity. Membrane hyperpolarization, caused by lowering the extracellular concentration of potassium or blocking Ca2+ influx in SCN cultures by lowering [Ca2+], reversibly abolished the rhythmic expression of Per1. In addition, the amplitude of Per1 expression was markedly decreased by voltage-gated Ca2+ channel antagonists. A similar result was observed for mouse Per1 and PER2. Together, these results strongly suggest that a transmembrane Ca2+ flux is necessary for sustained molecular rhythmicity in the SCN. We propose that periodic Ca2+ influx, resulting from circadian variations in membrane potential, is a critical process for circadian pacemaker function.
Files in This Item:
T200505054.pdf Download
DOI
10.1523/JNEUROSCI.2211-05.2005
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kwak, Yong Ho(곽용호)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114981
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