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생쥐 Pilocarpine 모델에서 중첩경련으로 유발한 해마신경세포 사멸과 이끼섬유발아에 대한 MMP-9의 역할

DC FieldValueLanguage
dc.contributor.author조양제-
dc.contributor.author주민경-
dc.contributor.author허경-
dc.contributor.author김경환-
dc.contributor.author김현우-
dc.contributor.author이병인-
dc.contributor.author조경주-
dc.date.accessioned2015-08-26T16:42:58Z-
dc.date.available2015-08-26T16:42:58Z-
dc.date.issued2005-
dc.identifier.issn1226-6965-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/114959-
dc.description.abstractPurpose: Matrix metalloproteinases (MMPs) have been known to participate in various pathologic situations by modulating extracellular matrix. Although MMP-9 upregulation has been reported in some experimental seizure models, the exact role of MMP-9 in hippocampal cell death during epileptogenesis and subsequent mossy fiber sprouting (MFS) is not clear. Here, we investigated the role of MMP-9 on hippocampal cell death and MFS after pilocarpine-induced status epilepticus (SE) in mice, using highly specific hydroxamic MMP-9 inhibitor. Methods: SE was induced by intraperitoneal pilocarpine administration in adult male C57BL/6 mice. MMP-9 specific inhibitor was administered intracerebroventrically 3 h after pilocarpine-induced SE. Expression and activation of MMP-9 were assessed by zymography and Western blot analysis. TdT-mediated UTP-biotin nick end labeling (TUNEL) and caspase-3 activity assay were also performed. MFS was investigated using Timm staining. Results: Increased expression and activation of MMP-9 after pilocarpine-induced SE were observed in zymography and Western blot analysis. MMP-9 specific inhibitor decreased MMP-9 activity in in situ zymography and hippocampal cell death in cresyl violet staining. DNA fragmentation and caspase-3 activity were also attenuated by MMP-9 specific inhibitor. Four months after pilocarpine-induced SE, MFS was evident in vehicle-treated mice; in contrast, MFS was barely observed in MMP-9 specific inhibitor-treated mice. Conclusions: This study suggests MMP-9 is associated with hippocampal cell death and MFS after pilocarpine-induced SE. Furthermore, the findings that MMP-9 specific inhibitor ameliorates cell death and MFS offers the possibility of MMP-9 specific hydroxamic inhibitor as novel therapeutic strategy to reduce hippocampal damage and epileptogenesis.-
dc.description.statementOfResponsibilityopen-
dc.format.extent119~128-
dc.relation.isPartOfJournal of Korean Epilepsy Society (대한간질학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHSeizures-
dc.subject.MESHGelatinase B-
dc.subject.MESHApoptosis-
dc.subject.MESHmossy fiber-
dc.subject.MESHhippocampal-
dc.subject.MESHanthranilic hydroxamic acid-
dc.title생쥐 Pilocarpine 모델에서 중첩경련으로 유발한 해마신경세포 사멸과 이끼섬유발아에 대한 MMP-9의 역할-
dc.title.alternativeThe Role of MMP-9 on the Hippocampal Neuronal Cell Death and Mossy Fiber Sprouting due to Pilocarpine-Induced Status Epilepticus in Mice-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthor주민경-
dc.contributor.googleauthor조양제-
dc.contributor.googleauthor이병인-
dc.contributor.googleauthor허경-
dc.contributor.googleauthor김경환-
dc.contributor.googleauthor김현정-
dc.contributor.googleauthor김현우-
dc.contributor.googleauthor이두재-
dc.contributor.googleauthor조경주-
dc.identifier.doiOAK-2005-02217-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03851-
dc.contributor.localIdA03950-
dc.contributor.localIdA04341-
dc.contributor.localIdA00310-
dc.contributor.localIdA02797-
dc.contributor.localIdA03804-
dc.contributor.localIdA01125-
dc.relation.journalcodeJ01509-
dc.subject.keywordSeizures-
dc.subject.keywordGelatinase B-
dc.subject.keywordApoptosis-
dc.subject.keywordmossy fiber-
dc.subject.keywordhippocampal-
dc.subject.keywordanthranilic hydroxamic acid-
dc.contributor.alternativeNameCho, Yang Je-
dc.contributor.alternativeNameChu, Min Kyung-
dc.contributor.alternativeNameHeo, Kyoung-
dc.contributor.alternativeNameKim, Gyung Whan-
dc.contributor.alternativeNameKim, Hyun Woo-
dc.contributor.alternativeNameLee, Byung In-
dc.contributor.alternativeNameCho, Kyuong Joo-
dc.contributor.affiliatedAuthorCho, Yang Je-
dc.contributor.affiliatedAuthorChu, Min Kyung-
dc.contributor.affiliatedAuthorHeo, Kyoung-
dc.contributor.affiliatedAuthorKim, Gyung Whan-
dc.contributor.affiliatedAuthorLee, Byung In-
dc.contributor.affiliatedAuthorCho, Kyuong Joo-
dc.contributor.affiliatedAuthorKim, Hyun Woo-
dc.rights.accessRightsfree-
dc.citation.volume9-
dc.citation.number2-
dc.citation.startPage119-
dc.citation.endPage128-
dc.identifier.bibliographicCitationJournal of Korean Epilepsy Society (대한간질학회지), Vol.9(2) : 119-128, 2005-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers

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