Cited 32 times in
Immunoglobulin E reactivity of recombinant allergen Tyr p 13 from Tyrophagus putrescentiae homologous to fatty acid binding protein
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 홍천수 | - |
dc.contributor.author | 김규언 | - |
dc.contributor.author | 박중원 | - |
dc.contributor.author | 용태순 | - |
dc.contributor.author | 이재식 | - |
dc.contributor.author | 이한일 | - |
dc.contributor.author | 정경용 | - |
dc.date.accessioned | 2015-08-26T16:41:47Z | - |
dc.date.available | 2015-08-26T16:41:47Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1071-412X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114925 | - |
dc.description.abstract | The storage mite, Tyrophagus putrescentiae, is one of the important causes of allergic disorders. Fifteen allergenic components were demonstrated in storage mite by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting, but only the group 2 allergen Tyr p 2 has been cloned and characterized. In this study, we attempted to identify and characterize new allergens from T. putrescentiae, which is a dominant species of storage mite in Korea. Expressed sequence tags were analyzed to identify possible storage mite allergens, and the cDNA sequence encoding a protein homologous to fatty acid binding protein, a mite group 13 allergen, was identified and named Tyr p 13. Its deduced amino acid sequence showed 61.1 to 85.3% identity with other mite group 13 allergens. The recombinant protein was expressed in Escherichia coli using a pET 28b vector system, and its allergenicity was investigated by enzyme-linked immunosorbent assay (ELISA). The recombinant allergen was detected in 5 of 78 (6.4%) T. putrescentiae-positive sera tested, and it inhibited 61.9% of immunoglobulin E binding to crude extract at an inhibitor concentration of 10 mug/ml by inhibition ELISA using serum from the patient who showed the strongest reaction by ELISA. In this study, a novel allergen was identified in T. putrescentiae. This allergen could be helpful for more-detailed characterizations of storage mite allergy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 581~585 | - |
dc.relation.isPartOf | CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acaridae/immunology* | - |
dc.subject.MESH | Adolescent | - |
dc.subject.MESH | Allergens/chemistry | - |
dc.subject.MESH | Allergens/immunology* | - |
dc.subject.MESH | Allergens/isolation & purification | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Carrier Proteins | - |
dc.subject.MESH | Child | - |
dc.subject.MESH | Child, Preschool | - |
dc.subject.MESH | Cloning, Molecular | - |
dc.subject.MESH | Fatty Acid-Binding Proteins | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypersensitivity/diagnosis | - |
dc.subject.MESH | Immunoglobulin E/immunology* | - |
dc.subject.MESH | Korea | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Proteins/chemistry* | - |
dc.subject.MESH | Proteins/isolation & purification | - |
dc.subject.MESH | Recombinant Proteins | - |
dc.subject.MESH | Sequence Homology, Nucleic Acid | - |
dc.title | Immunoglobulin E reactivity of recombinant allergen Tyr p 13 from Tyrophagus putrescentiae homologous to fatty acid binding protein | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Kyoung Yong Jeong | - |
dc.contributor.googleauthor | Woo Kyung Kim | - |
dc.contributor.googleauthor | Tai-Soon Yong | - |
dc.contributor.googleauthor | Han-Il Ree | - |
dc.contributor.googleauthor | Chein-Soo Hong | - |
dc.contributor.googleauthor | Jung Won Park | - |
dc.contributor.googleauthor | Kyu-Earn Kim | - |
dc.contributor.googleauthor | In-Yong Lee | - |
dc.contributor.googleauthor | Jongweon Lee | - |
dc.contributor.googleauthor | Jae Sik Lee | - |
dc.identifier.doi | 10.1128/CDLI.12.5.581-585.2005 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00327 | - |
dc.contributor.localId | A01681 | - |
dc.contributor.localId | A02424 | - |
dc.contributor.localId | A03077 | - |
dc.contributor.localId | A03279 | - |
dc.contributor.localId | A04448-1 | - |
dc.contributor.localId | A03572 | - |
dc.relation.journalcode | J00547 | - |
dc.identifier.eissn | 1098-6588 | - |
dc.identifier.pmid | 15879018 | - |
dc.subject.keyword | 15879018 | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.contributor.alternativeName | Kim, Kyu Earn | - |
dc.contributor.alternativeName | Park, Jung Won | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.alternativeName | Lee, Jae Sik | - |
dc.contributor.alternativeName | Ree, Han Il | - |
dc.contributor.alternativeName | Jeong, Kyoung Yong | - |
dc.contributor.affiliatedAuthor | Kim, Kyu Earn | - |
dc.contributor.affiliatedAuthor | Park, Jung Won | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Lee, Jae Sik | - |
dc.contributor.affiliatedAuthor | Ree, Han Il | - |
dc.contributor.affiliatedAuthor | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Jeong, Kyoung Yong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 12 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 581 | - |
dc.citation.endPage | 585 | - |
dc.identifier.bibliographicCitation | CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY , Vol.12(5) : 581-585, 2005 | - |
dc.identifier.rimsid | 39315 | - |
dc.type.rims | ART | - |
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