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Immunoglobulin E reactivity of recombinant allergen Tyr p 13 from Tyrophagus putrescentiae homologous to fatty acid binding protein

DC Field Value Language
dc.contributor.author홍천수-
dc.contributor.author김규언-
dc.contributor.author박중원-
dc.contributor.author용태순-
dc.contributor.author이재식-
dc.contributor.author이한일-
dc.contributor.author정경용-
dc.date.accessioned2015-08-26T16:41:47Z-
dc.date.available2015-08-26T16:41:47Z-
dc.date.issued2005-
dc.identifier.issn1071-412X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/114925-
dc.description.abstractThe storage mite, Tyrophagus putrescentiae, is one of the important causes of allergic disorders. Fifteen allergenic components were demonstrated in storage mite by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting, but only the group 2 allergen Tyr p 2 has been cloned and characterized. In this study, we attempted to identify and characterize new allergens from T. putrescentiae, which is a dominant species of storage mite in Korea. Expressed sequence tags were analyzed to identify possible storage mite allergens, and the cDNA sequence encoding a protein homologous to fatty acid binding protein, a mite group 13 allergen, was identified and named Tyr p 13. Its deduced amino acid sequence showed 61.1 to 85.3% identity with other mite group 13 allergens. The recombinant protein was expressed in Escherichia coli using a pET 28b vector system, and its allergenicity was investigated by enzyme-linked immunosorbent assay (ELISA). The recombinant allergen was detected in 5 of 78 (6.4%) T. putrescentiae-positive sera tested, and it inhibited 61.9% of immunoglobulin E binding to crude extract at an inhibitor concentration of 10 mug/ml by inhibition ELISA using serum from the patient who showed the strongest reaction by ELISA. In this study, a novel allergen was identified in T. putrescentiae. This allergen could be helpful for more-detailed characterizations of storage mite allergy.-
dc.description.statementOfResponsibilityopen-
dc.format.extent581~585-
dc.relation.isPartOfCLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAcaridae/immunology*-
dc.subject.MESHAdolescent-
dc.subject.MESHAllergens/chemistry-
dc.subject.MESHAllergens/immunology*-
dc.subject.MESHAllergens/isolation & purification-
dc.subject.MESHAnimals-
dc.subject.MESHBase Sequence-
dc.subject.MESHCarrier Proteins-
dc.subject.MESHChild-
dc.subject.MESHChild, Preschool-
dc.subject.MESHCloning, Molecular-
dc.subject.MESHFatty Acid-Binding Proteins-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHHypersensitivity/diagnosis-
dc.subject.MESHImmunoglobulin E/immunology*-
dc.subject.MESHKorea-
dc.subject.MESHMale-
dc.subject.MESHProteins/chemistry*-
dc.subject.MESHProteins/isolation & purification-
dc.subject.MESHRecombinant Proteins-
dc.subject.MESHSequence Homology, Nucleic Acid-
dc.titleImmunoglobulin E reactivity of recombinant allergen Tyr p 13 from Tyrophagus putrescentiae homologous to fatty acid binding protein-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorKyoung Yong Jeong-
dc.contributor.googleauthorWoo Kyung Kim-
dc.contributor.googleauthorTai-Soon Yong-
dc.contributor.googleauthorHan-Il Ree-
dc.contributor.googleauthorChein-Soo Hong-
dc.contributor.googleauthorJung Won Park-
dc.contributor.googleauthorKyu-Earn Kim-
dc.contributor.googleauthorIn-Yong Lee-
dc.contributor.googleauthorJongweon Lee-
dc.contributor.googleauthorJae Sik Lee-
dc.identifier.doi10.1128/CDLI.12.5.581-585.2005-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00327-
dc.contributor.localIdA01681-
dc.contributor.localIdA02424-
dc.contributor.localIdA03077-
dc.contributor.localIdA03279-
dc.contributor.localIdA04448-1-
dc.contributor.localIdA03572-
dc.relation.journalcodeJ00547-
dc.identifier.eissn1098-6588-
dc.identifier.pmid15879018-
dc.subject.keyword15879018-
dc.contributor.alternativeNameHong, Chein Soo-
dc.contributor.alternativeNameKim, Kyu Earn-
dc.contributor.alternativeNamePark, Jung Won-
dc.contributor.alternativeNameYong, Tai Soon-
dc.contributor.alternativeNameLee, Jae Sik-
dc.contributor.alternativeNameRee, Han Il-
dc.contributor.alternativeNameJeong, Kyoung Yong-
dc.contributor.affiliatedAuthorKim, Kyu Earn-
dc.contributor.affiliatedAuthorPark, Jung Won-
dc.contributor.affiliatedAuthorYong, Tai Soon-
dc.contributor.affiliatedAuthorLee, Jae Sik-
dc.contributor.affiliatedAuthorRee, Han Il-
dc.contributor.affiliatedAuthorHong, Chein Soo-
dc.contributor.affiliatedAuthorJeong, Kyoung Yong-
dc.rights.accessRightsfree-
dc.citation.volume12-
dc.citation.number5-
dc.citation.startPage581-
dc.citation.endPage585-
dc.identifier.bibliographicCitationCLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY , Vol.12(5) : 581-585, 2005-
dc.identifier.rimsid39315-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers

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