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NADPH oxidase mediates interleukin-6 expression in cerulein-stimulated pancreatic acinar cells

DC Field Value Language
dc.contributor.author김경환-
dc.contributor.author김혜영-
dc.contributor.author유지훈-
dc.contributor.author임주원-
dc.date.accessioned2015-08-26T16:37:25Z-
dc.date.available2015-08-26T16:37:25Z-
dc.date.issued2005-
dc.identifier.issn1357-2725-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/114806-
dc.description.abstractNADPH oxidase produces a large amount of reactive oxygen species (ROS) mainly in phagocytic cells. ROS are involved in NF-κB activation, cytokine expression and thus, pathogenesis of pancreatitis. However, the source of ROS in pancreatic acinar cells has not been clarified. Cerulein rapidly induces acute and edematous form of pancreatitis. We investigated whether pancreatic acinar cells contain NADPH oxidase, and whether NADPH oxidase mediates interleukin-6 (IL-6) in pancreatic acinar AR42J cells stimulated with cerulein. Expression of NADPH oxidase subunits and NADPH oxidase activity were determined in the cells by immunofluorescence staining and lucigenin luminescence, respectively. Oxidant-sensitive nuclear transcription factor NF-κB activation was monitored by electrophoretic mobility shift assay. IL-6 expression was determined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbant assay. NADPH oxidase inhibitor diphenylene iodonium (DPI), antioxidant rebamipide, and antisense oligonucleotides (AS ODNs) for NADPH oxidase subunits p22phox and p47phox were used to determine the involvement of NADPH oxidase in NF-κB activation and IL-6 expression in AR42J cells. As a result, pancreatic acinar AR42J cells constitutively express NADPH oxidase subunits p67phox and p47phox in the cytosol and Nox1 and p22phox in the membrane. Cerulein-stimulated NADPH oxidase activity and induced NF-κB activation and IL-6 expression in AR42J cells. Treatment of DPI or rebamipide and transfection of AS ODNs for NADPH oxidase subunits suppressed cerulein-induced NF-κB activation and IL-6 expression compared to S ODNs. In conclusion, NADPH oxidase may mediate the expression of inflammatory cytokines by stimulating NF-κB activation in pancreatic acinar cells during the course of pancreatitis.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1458~1469-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCeruletide/toxicity*-
dc.subject.MESHElectrophoretic Mobility Shift Assay-
dc.subject.MESHEnzyme Activation-
dc.subject.MESHInterleukin-6/biosynthesis*-
dc.subject.MESHNADPH Oxidases/antagonists & inhibitors-
dc.subject.MESHNADPH Oxidases/metabolism*-
dc.subject.MESHNF-kappa B/metabolism*-
dc.subject.MESHOnium Compounds/pharmacology-
dc.subject.MESHPancreas/enzymology-
dc.subject.MESHPancreas/metabolism*-
dc.subject.MESHProtein Subunits/antagonists & inhibitors-
dc.subject.MESHProtein Subunits/metabolism-
dc.subject.MESHRats-
dc.subject.MESHReactive Oxygen Species/metabolism*-
dc.titleNADPH oxidase mediates interleukin-6 expression in cerulein-stimulated pancreatic acinar cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorJi Hoon Yu-
dc.contributor.googleauthorJoo Weon Lim-
dc.contributor.googleauthorKyung Hwan Kim-
dc.contributor.googleauthorHyeyoung Kim-
dc.identifier.doi10.1016/j.biocel.2005.02.004-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03405-
dc.contributor.localIdA00311-
dc.contributor.localIdA01175-
dc.contributor.localIdA02523-
dc.relation.journalcodeJ01089-
dc.identifier.eissn1878-5875-
dc.identifier.pmid15833277-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1357272505000385-
dc.subject.keywordNADPH oxidase-
dc.subject.keywordNF-κB-
dc.subject.keywordIL-6-
dc.subject.keywordPancreatic acinar cells-
dc.subject.keywordCerulein-
dc.contributor.alternativeNameKim, Kyung Hwan-
dc.contributor.alternativeNameKim, Hye Young-
dc.contributor.alternativeNameYu, Ji Hoon-
dc.contributor.alternativeNameLim, Joo Weon-
dc.contributor.affiliatedAuthorLim, Joo Weon-
dc.contributor.affiliatedAuthorKim, Kyung Hwan-
dc.contributor.affiliatedAuthorKim, Hye Young-
dc.contributor.affiliatedAuthorYu, Ji Hoon-
dc.rights.accessRightsnot free-
dc.citation.volume37-
dc.citation.number7-
dc.citation.startPage1458-
dc.citation.endPage1469-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol.37(7) : 1458-1469, 2005-
dc.identifier.rimsid38489-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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