Cited 10 times in
Effect of PK11195, a peripheral benzodiazepine receptor agonist, on insulinoma cell death and insulin secretion
DC Field | Value | Language |
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dc.contributor.author | 장인익 | - |
dc.date.accessioned | 2015-08-26T16:34:48Z | - |
dc.date.available | 2015-08-26T16:34:48Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1360-8185 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114735 | - |
dc.description.abstract | Functional role of peripheral benzodiazepine receptor on mitochondrial membrane in apoptosis and insulin secretion from insulinoma cells was studied. A prototypic peripheral benzodiazepine receptor agonist PK11195 induced insulinoma cell apoptosis, while a central benzodiazepine receptor agonist did not. Death of insulinoma cells by PK11195 was inhibited by cyclosporin A,{ a blocker of mitochondrial permeability transition pore}. Caspase inhibitors further inhibited MIN6N8 cell death. PK11195 induced dissipation of mitochondrial potential and cytochrome c translocation to cytoplasm. PK11195 induced an increase in cytoplasmic [Ca2 +], which was reversed by cyclosporin A. Rhod-2 staining showed decreased mitochondrial [Ca2 +] after PK11195 treatment. PK11195 potentiated glucose-induced insulin secretion probably due to the increased cytoplasmic [Ca2 +]. Calpain was activated following Ca2 + release, and calpain inhibitors attenuated death of insulinoma cells by PK11195. These results suggest that PK11195 induces mitochondrial potential loss, cytochrome c translocation, increased insulin secretion in conjunction with an increase in cytoplasmic [Ca2 +] and calpain activation, which collectively leads to apoptosis of insulinoma cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 537~544 | - |
dc.relation.isPartOf | APOPTOSIS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis/drug effects* | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Calpain/metabolism | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | GABA-A Receptor Agonists* | - |
dc.subject.MESH | Insulin/metabolism* | - |
dc.subject.MESH | Insulin Secretion | - |
dc.subject.MESH | Insulinoma/physiopathology* | - |
dc.subject.MESH | Intracellular Membranes/drug effects | - |
dc.subject.MESH | Intracellular Membranes/physiology | - |
dc.subject.MESH | Isoquinolines/pharmacology* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mitochondria/drug effects | - |
dc.subject.MESH | Mitochondria/physiology | - |
dc.subject.MESH | Permeability | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.title | Effect of PK11195, a peripheral benzodiazepine receptor agonist, on insulinoma cell death and insulin secretion | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | S. Y. Park | - |
dc.contributor.googleauthor | N. Cho | - |
dc.contributor.googleauthor | M.-S. Lee | - |
dc.contributor.googleauthor | S. J. Kim | - |
dc.contributor.googleauthor | K.-W. Kim | - |
dc.contributor.googleauthor | M.-K. Lee | - |
dc.contributor.googleauthor | Y.-K. Min | - |
dc.contributor.googleauthor | J.-H. Chung | - |
dc.contributor.googleauthor | I. Chang | - |
dc.identifier.doi | 10.1007/s10495-005-1884-1 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03461 | - |
dc.relation.journalcode | J00195 | - |
dc.identifier.eissn | 1573-675X | - |
dc.identifier.pmid | 15909116 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs10495-005-1884-1 | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | benzodiazepine receptor | - |
dc.subject.keyword | calcium | - |
dc.subject.keyword | insulin | - |
dc.subject.keyword | mitochondria | - |
dc.contributor.alternativeName | Chang, In Ik | - |
dc.contributor.affiliatedAuthor | Chang, In Ik | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 10 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 537 | - |
dc.citation.endPage | 544 | - |
dc.identifier.bibliographicCitation | APOPTOSIS, Vol.10(3) : 537-544, 2005 | - |
dc.identifier.rimsid | 46086 | - |
dc.type.rims | ART | - |
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