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Effect of PK11195, a peripheral benzodiazepine receptor agonist, on insulinoma cell death and insulin secretion

DC FieldValueLanguage
dc.contributor.author장인익-
dc.date.accessioned2015-08-26T16:34:48Z-
dc.date.available2015-08-26T16:34:48Z-
dc.date.issued2005-
dc.identifier.issn1360-8185-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/114735-
dc.description.abstractFunctional role of peripheral benzodiazepine receptor on mitochondrial membrane in apoptosis and insulin secretion from insulinoma cells was studied. A prototypic peripheral benzodiazepine receptor agonist PK11195 induced insulinoma cell apoptosis, while a central benzodiazepine receptor agonist did not. Death of insulinoma cells by PK11195 was inhibited by cyclosporin A,{ a blocker of mitochondrial permeability transition pore}. Caspase inhibitors further inhibited MIN6N8 cell death. PK11195 induced dissipation of mitochondrial potential and cytochrome c translocation to cytoplasm. PK11195 induced an increase in cytoplasmic [Ca2 +], which was reversed by cyclosporin A. Rhod-2 staining showed decreased mitochondrial [Ca2 +] after PK11195 treatment. PK11195 potentiated glucose-induced insulin secretion probably due to the increased cytoplasmic [Ca2 +]. Calpain was activated following Ca2 + release, and calpain inhibitors attenuated death of insulinoma cells by PK11195. These results suggest that PK11195 induces mitochondrial potential loss, cytochrome c translocation, increased insulin secretion in conjunction with an increase in cytoplasmic [Ca2 +] and calpain activation, which collectively leads to apoptosis of insulinoma cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent537~544-
dc.relation.isPartOfAPOPTOSIS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHCalcium/metabolism-
dc.subject.MESHCalpain/metabolism-
dc.subject.MESHEnzyme Activation-
dc.subject.MESHGABA-A Receptor Agonists*-
dc.subject.MESHInsulin/metabolism*-
dc.subject.MESHInsulin Secretion-
dc.subject.MESHInsulinoma/physiopathology*-
dc.subject.MESHIntracellular Membranes/drug effects-
dc.subject.MESHIntracellular Membranes/physiology-
dc.subject.MESHIsoquinolines/pharmacology*-
dc.subject.MESHMice-
dc.subject.MESHMitochondria/drug effects-
dc.subject.MESHMitochondria/physiology-
dc.subject.MESHPermeability-
dc.subject.MESHTumor Cells, Cultured-
dc.titleEffect of PK11195, a peripheral benzodiazepine receptor agonist, on insulinoma cell death and insulin secretion-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학)-
dc.contributor.googleauthorS. Y. Park-
dc.contributor.googleauthorN. Cho-
dc.contributor.googleauthorM.-S. Lee-
dc.contributor.googleauthorS. J. Kim-
dc.contributor.googleauthorK.-W. Kim-
dc.contributor.googleauthorM.-K. Lee-
dc.contributor.googleauthorY.-K. Min-
dc.contributor.googleauthorJ.-H. Chung-
dc.contributor.googleauthorI. Chang-
dc.identifier.doi10.1007/s10495-005-1884-1-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03461-
dc.relation.journalcodeJ00195-
dc.identifier.eissn1573-675X-
dc.identifier.pmid15909116-
dc.identifier.urlhttp://link.springer.com/article/10.1007%2Fs10495-005-1884-1-
dc.subject.keywordapoptosis-
dc.subject.keywordbenzodiazepine receptor-
dc.subject.keywordcalcium-
dc.subject.keywordinsulin-
dc.subject.keywordmitochondria-
dc.contributor.alternativeNameChang, In Ik-
dc.contributor.affiliatedAuthorChang, In Ik-
dc.rights.accessRightsnot free-
dc.citation.volume10-
dc.citation.number3-
dc.citation.startPage537-
dc.citation.endPage544-
dc.identifier.bibliographicCitationAPOPTOSIS, Vol.10(3) : 537-544, 2005-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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