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Ca2+ Activates Cystic Fibrosis Transmembrane Conductance Regulator- and Cl−-dependent HCOFormula Transport in Pancreatic Duct Cells

DC FieldValueLanguage
dc.contributor.author안우인-
dc.contributor.author이민구-
dc.contributor.author이진아-
dc.contributor.author김경환-
dc.contributor.author안덕선-
dc.date.accessioned2015-07-15T17:19:08Z-
dc.date.available2015-07-15T17:19:08Z-
dc.date.issued2003-
dc.identifier.issn0021-9258-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/114667-
dc.description.abstractPancreatic duct cells secrete bicarbonate-rich fluids, which are important for maintaining the patency of pancreatic ductal trees as well as intestinal digestive function. The bulk of bicarbonate secretion in the luminal membrane of duct cells is mediated by a Cl(-)-dependent mechanism (Cl(-)/HCO(3)(-) exchange), and we previously reported that the mechanism is CFTR-dependent and cAMP-activated (Lee, M. G., Choi, J. Y., Luo, X., Strickland, E., Thomas, P. J., and Muallem, S. (1999) J. Biol. Chem. 274, 14670-14677). In the present study, we provide comprehensive evidence that calcium signaling also activates the same CFTR- and Cl(-)-dependent HCO(3)(-) transport. ATP and trypsin evoked intracellular calcium signaling in pancreatic duct-derived cells through the activation of purinergic and protease-activated receptors, respectively. Cl(-)/HCO(3)(-) exchange activity was measured by recording pH(i) in response to [Cl(-)](o) changes of the perfusate. In perfusate containing high concentrations of K(+), which blocks Cl(-) movement through electrogenic or K(+)-coupled pathways, ATP and trypsin highly stimulated luminal Cl(-)/HCO(3)(-) exchange activity in CAPAN-1 cells expressing wild-type CFTR, but not in CFPAC-1 cells that have defective (DeltaF508) CFTR. Notably, adenoviral transfection of wild-type CFTR in CFPAC-1 cells completely restored the stimulatory effect of ATP on luminal Cl(-)/HCO(3)(-) exchange. In addition, the chelation of intracellular calcium by 1,2-bis(2-aminophenoxy)ethane-N,N,N,N'-tetraacetic acid (BAPTA) treatment abolished the effect of calcium agonists on luminal Cl(-)/HCO(3)(-) exchange. These results provide a molecular basis for calcium-induced bicarbonate secretion in pancreatic duct cells and highlight the importance of CFTR in epithelial bicarbonate secretion induced by various stimuli.-
dc.description.statementOfResponsibilityopen-
dc.format.extent200~207-
dc.relation.isPartOfJOURNAL OF BIOLOGICAL CHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleCa2+ Activates Cystic Fibrosis Transmembrane Conductance Regulator- and Cl−-dependent HCOFormula Transport in Pancreatic Duct Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Physiology (생리학)-
dc.contributor.googleauthorWan Namkung-
dc.contributor.googleauthorJin Ah Lee-
dc.contributor.googleauthorMin Goo Lee-
dc.contributor.googleauthorKyung Hwan Kim-
dc.contributor.googleauthorDuk Sun Ahn-
dc.contributor.googleauthorSung Won Kwon-
dc.contributor.googleauthorWonSun Han-
dc.contributor.googleauthorWooin Ahn-
dc.identifier.doi10.1074/jbc.M207199200-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02250-
dc.contributor.localIdA02781-
dc.contributor.localIdA03228-
dc.contributor.localIdA00311-
dc.contributor.localIdA02223-
dc.relation.journalcodeJ01258-
dc.identifier.eissn1083-351X-
dc.identifier.pmid10.1074/jbc.M207199200-
dc.contributor.alternativeNameAhn, Woo In-
dc.contributor.alternativeNameLee, Min Goo-
dc.contributor.alternativeNameLee, Jin Ah-
dc.contributor.alternativeNameKim, Kyung Hwan-
dc.contributor.alternativeNameAhn, Duk Sun-
dc.contributor.affiliatedAuthorAhn, Woo In-
dc.contributor.affiliatedAuthorLee, Min Goo-
dc.contributor.affiliatedAuthorLee, Jin Ah-
dc.contributor.affiliatedAuthorKim, Kyung Hwan-
dc.contributor.affiliatedAuthorAhn, Duk Sun-
dc.rights.accessRightsfree-
dc.citation.volume278-
dc.citation.number1-
dc.citation.startPage200-
dc.citation.endPage207-
dc.identifier.bibliographicCitationJOURNAL OF BIOLOGICAL CHEMISTRY, Vol.278(1) : 200-207, 2003-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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