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Acetyl-CoA carboxylase beta gene is regulated by sterol regulatory element-binding protein-1 in liver

 So-Young Oh  ;  Sahng-Kyoo Park  ;  Kyung-Sup Kim  ;  Sahng-Wook Park  ;  Yong-Ho Ahn  ;  Jae-Woo Kim 
 Journal of Biological Chemistry, Vol.278(31) : 28410-28417, 2003 
Journal Title
 Journal of Biological Chemistry 
Issue Date
Acetyl-CoA Carboxylase/genetics* ; Animals ; Base Sequence ; Binding Sites ; CCAAT-Enhancer-Binding Proteins/metabolism ; CCAAT-Enhancer-Binding Proteins/pharmacology* ; DNA/chemistry ; DNA-Binding Proteins/metabolism ; DNA-Binding Proteins/pharmacology* ; Diet ; Dietary Carbohydrates/administration & dosage ; Gene Expression Regulation, Enzymologic/drug effects* ; Humans ; Isoenzymes/genetics* ; Liver/enzymology* ; Luciferases/genetics ; Male ; Nutritional Status ; Promoter Regions, Genetic/genetics ; RNA, Messenger/analysis ; Rats ; Rats, Sprague-Dawley ; Response Elements ; Sequence Alignment ; Sterol Regulatory Element Binding Protein 1 ; Transcription Factors* ; Transfection
Acetyl-CoA carboxylase (ACC) exists as two major isoforms originated from separate genes: ACCalpha (or ACC1) and ACCbeta (or ACC2). Previous data revealed that ACCbeta has two forms of mRNA with different 5'-untranslated regions derived by different usage of promoters, I and II, in human. In this study, we revealed that ACCbeta expression in liver is markedly stimulated by food intake at the transcriptional level. In the process of this induction in rat liver, promoter II plays the major role in regulating the expression of ACCbeta gene. The transient transfection with promoter II-luciferase reporters elucidated that the region from -93 to -38 nucleotides is important for the responsiveness to sterol regulatory element-binding protein-1 (SREBP-1), which is known to be the principle mediator for the stimulation of gene transcriptions by insulin and diet. The Sp1-binding site (-71 to -66) and neighboring two conserved SREs (-62 to -44) play a critical role in the stimulation of ACCbeta gene expression by SREBP-1. In vivo chromatin immunoprecipitation assay revealed that SREBP-1 directly bound to ACCbeta promoter II in liver, and its binding was regulated by the diet. This study provides evidence that ACCbeta expression in liver is regulated at the transcriptional level by the direct interaction of SREBP-1 with promoter II.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jae Woo(김재우) ORCID logo https://orcid.org/0000-0001-5456-9495
Park, Sahng Wook(박상욱) ORCID logo https://orcid.org/0000-0002-9594-7074
Ahn, Yong Ho(안용호) ORCID logo https://orcid.org/0000-0002-4133-0757
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