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배양된 골수 간엽 간세포에서 골형성의 유도 및 그 표식 인자의 발현

Other Titles
 Induction of Osteogenesis from Cultured Mesenchymal Stem Cells and Their Marker Expression 
 석경수  ;  김현우  ;  한수봉 
 Journal of Korean Musculoskeletal Tissue Transplantation Society (대한골ㆍ연부조직이식학회지), Vol.3(1) : 26-35, 2003 
Journal Title
 Journal of Korean Musculoskeletal Tissue Transplantation Society (대한골ㆍ연부조직이식학회지) 
Issue Date
Bone marrow ; Osteogenesis ; Mesenchymal stem cell
Bone marrow ; Osteogenesis ; Mesenchymal stem cell
Purpose: To induce osteogenesis from culuted mesenchymal stem cell isolated from human bone marrow and to detect the marker of osteogenesis to develop cell therapy method Materials & Methods: Mesenchymal stem cell (MSC) was isolated form human bone marrow and cultured. In vitro and in vivo induction of osteogenesis was performed. And histological, immunohistochemical and molecular biological study was performed to confirm osteogenesis. Results: Osteogenesis induced MSCs were strongly stained by ALP immunochemical stain. Von Kossa stain showed black meneral deposition. Tetracyclin labeling revealed new bone formation. RT-PCR for osteocalcin m-RNA showed increased osteocalcin m-RNA. H-E stain showed penetration of cellular component into the pores of collagraft strip and showed formation of osteoid. Conclusion: Based on the results, osteogenesis could be induced from cultured mesenchymal stem cell isolated from human bone marrow. Expression of markers for osteogenesis was con-firmed by histologic, immunohistochemical, and molecular biologic method.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Woo(김현우) ORCID logo https://orcid.org/0000-0001-8576-1877
Suk, Kyung Soo(석경수) ORCID logo https://orcid.org/0000-0003-0633-2658
Hahn, Soo Bong(한수봉)
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