배양된 골수 간엽 간세포에서 골형성의 유도 및 그 표식 인자의 발현

Abstract

Purpose: To induce osteogenesis from culuted mesenchymal stem cell isolated from human bone marrow and to detect the marker of osteogenesis to develop cell therapy method Materials & Methods: Mesenchymal stem cell (MSC) was isolated form human bone marrow and cultured. In vitro and in vivo induction of osteogenesis was performed. And histological, immunohistochemical and molecular biological study was performed to confirm osteogenesis. Results: Osteogenesis induced MSCs were strongly stained by ALP immunochemical stain. Von Kossa stain showed black meneral deposition. Tetracyclin labeling revealed new bone formation. RT-PCR for osteocalcin m-RNA showed increased osteocalcin m-RNA. H-E stain showed penetration of cellular component into the pores of collagraft strip and showed formation of osteoid. Conclusion: Based on the results, osteogenesis could be induced from cultured mesenchymal stem cell isolated from human bone marrow. Expression of markers for osteogenesis was con-firmed by histologic, immunohistochemical, and molecular biologic method.