Cited 4 times in
Partial inhibition of SERCA is responsible for extracellular Ca2 dependence of AlF4-induced [Ca2 ]i oscillations in rat pancreatic acinar cells
DC Field | Value | Language |
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dc.contributor.author | 문석준 | - |
dc.contributor.author | 서정택 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 이승일 | - |
dc.date.accessioned | 2015-07-15T17:15:24Z | - |
dc.date.available | 2015-07-15T17:15:24Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0363-6143 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114544 | - |
dc.description.abstract | AlF4-is known to generate oscillations in intracellular Ca2+ concentration ([Ca2+]i) by activating G proteins in many cell types. However, in rat pancreatic acinar cells, AlF4--evoked [Ca2+]i oscillations were reported to be dependent on extracellular Ca2+, which contrasts with the [Ca2+]i oscillations induced by cholecystokinin (CCK). Therefore, we investigated the mechanisms by which AlF4- generates extracellular Ca2+-dependent [Ca2+]i oscillations in rat pancreatic acinar cells. AlF4(-)-induced [Ca2+]i oscillations were stopped rapidly by the removal of extracellular Ca2+ and were abolished on the addition of 20 mM caffeine and 2 microM thapsigargin, indicating that Ca2+ influx plays a crucial role in maintenance of the oscillations and that an inositol 1,4,5-trisphosphate-sensitive Ca2+ store is also required. The amount of Ca2+ in the intracellular Ca2+ store was decreased as the AlF4--induced [Ca2+]i oscillations continued. Measurement of 45Ca2+ influx into isolated microsomes revealed that AlF4-directly inhibited sarco/endoplasmic reticulum Ca2+-ATPase (SERCA). The activity of plasma membrane Ca2+-ATPase during AlF4- stimulation was not significantly different from that during CCK stimulation. After partial inhibition of SERCA with 1 nM thapsigargin, 20 pM CCK-evoked [Ca2+]i oscillations were dependent on extracellular Ca2+. This study shows that AlF4- induces [Ca2+]i oscillations, probably by inositol 1,4,5-trisphosphate production via G protein activation but that these oscillations are strongly dependent on extracellular Ca2+ as a result of the partial inhibition of SERCA. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | C1142~C1149 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Aluminum Compounds/pharmacology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Calcium/physiology* | - |
dc.subject.MESH | Calcium Signaling/drug effects* | - |
dc.subject.MESH | Calcium Signaling/physiology | - |
dc.subject.MESH | Calcium-Transporting ATPases/antagonists & inhibitors* | - |
dc.subject.MESH | Calcium-Transporting ATPases/physiology* | - |
dc.subject.MESH | Extracellular Fluid/drug effects | - |
dc.subject.MESH | Extracellular Fluid/enzymology | - |
dc.subject.MESH | Fluorides/pharmacology* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Pancreas/cytology | - |
dc.subject.MESH | Pancreas/drug effects* | - |
dc.subject.MESH | Pancreas/enzymology* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Sarcoplasmic Reticulum Calcium-Transporting ATPases | - |
dc.title | Partial inhibition of SERCA is responsible for extracellular Ca2 dependence of AlF4-induced [Ca2 ]i oscillations in rat pancreatic acinar cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Seon Ah Chong | - |
dc.contributor.googleauthor | Soo Young Hong | - |
dc.contributor.googleauthor | Jeong Taeg Seo | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.contributor.googleauthor | Syng-Ill Lee | - |
dc.contributor.googleauthor | Jeong Mi An | - |
dc.contributor.googleauthor | Jeong-Hee Hong | - |
dc.contributor.googleauthor | Jee Won Park | - |
dc.contributor.googleauthor | Seok Jun Moon | - |
dc.identifier.doi | 10.1152/ajpcell.00566.2002 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01358 | - |
dc.contributor.localId | A01905 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02924 | - |
dc.relation.journalcode | J00102 | - |
dc.identifier.eissn | 1522-1563 | - |
dc.identifier.pmid | 12878491 | - |
dc.subject.keyword | 12878491 | - |
dc.contributor.alternativeName | Moon, Seok Jun | - |
dc.contributor.alternativeName | Seo, Jeong Taeg | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Lee, Syng Ill | - |
dc.contributor.affiliatedAuthor | Moon, Seok Jun | - |
dc.contributor.affiliatedAuthor | Seo, Jeong Taeg | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Lee, Syng Ill | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 285 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1142 | - |
dc.citation.endPage | 1149 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, Vol.285(5) : 1142-1149, 2003 | - |
dc.identifier.rimsid | 43919 | - |
dc.type.rims | ART | - |
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