Cited 14 times in
Analysis of plausible downstream target genes of Hoxc8 in F9 teratocarcinoma cells – Putative downstream target genes of Hoxc8
DC Field | Value | Language |
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dc.contributor.author | 권윤정 | - |
dc.contributor.author | 김명희 | - |
dc.contributor.author | 김병규 | - |
dc.date.accessioned | 2015-07-15T17:14:34Z | - |
dc.date.available | 2015-07-15T17:14:34Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0301-4851 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114516 | - |
dc.description.abstract | Although Hox genes are known to mediate developmental decisions involved in pattern formation during embryogenesis, it is still not well understood what Hox regulates. In order to analyze Hoxc8 downstream target genes, a stable cell line overexpressing Hoxc8 was established using F9 murine teratocarcinoma cells, proteom samples were analyzed by 2-DE, and compared with controls. The protein spots having differences more than 4 fold in intensity were selected, analyzed by MALDI-TOF, and grouped in terms of putative function; cytoskeleton and motility (vimentin, γ-actin, tropomyosin, and tubulin β-5 chain); folding, modification and degradation of protein (GRP78, proteasome subunit α type 5, 26S proteasome regulatory subunit p27 protein, and PDIR); metabolism (ATP synthase beta subunit, Pgam1, and CAII); transcription/translation factors and general nucleic acid binding proteins (RbAp46, PCNA, eEF-1-beta, and nucleophosmin). Although it may not be significant, 50% of the genes were located on chromosomes 2 and 3, suggesting the possibility of a non-random distribution of Hox downstream genes. Almost 50% of the genes analyzed showed some relation with Hox protein directly or indirectly; i.e., tubulin beta 5, EF-1 beta and PCNA have been reported to contain putative Hox binding regulatory sites and genes like vimentin, pgam1 and nucleophosmin to be regulated by RA, a potent modulator of Hox expression. These results altogether imply that proteom analysis could be a possible tool for the analysis of the potent Hox realizator genes, which provides a new insight into the function of Hox on pattern formation during embryogenesis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 141~148 | - |
dc.relation.isPartOf | MOLECULAR BIOLOGY REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Electrophoresis, Gel, Two-Dimensional | - |
dc.subject.MESH | Gene Expression Profiling* | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic* | - |
dc.subject.MESH | Genes, Reporter/genetics | - |
dc.subject.MESH | Homeodomain Proteins/genetics | - |
dc.subject.MESH | Homeodomain Proteins/metabolism* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Molecular Weight | - |
dc.subject.MESH | Neoplasm Proteins/analysis | - |
dc.subject.MESH | Neoplasm Proteins/genetics | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | - |
dc.subject.MESH | Substrate Specificity | - |
dc.subject.MESH | Teratocarcinoma/genetics* | - |
dc.subject.MESH | Teratocarcinoma/metabolism | - |
dc.title | Analysis of plausible downstream target genes of Hoxc8 in F9 teratocarcinoma cells – Putative downstream target genes of Hoxc8 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anatomy (해부학) | - |
dc.contributor.googleauthor | Yunjeong Kwon | - |
dc.contributor.googleauthor | Jeong Heon Ko | - |
dc.contributor.googleauthor | Myoung Hee Kim | - |
dc.contributor.googleauthor | Byungkyu Kim | - |
dc.identifier.doi | 10.1023/A:1024920418148 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00241 | - |
dc.contributor.localId | A00432 | - |
dc.contributor.localId | A00492 | - |
dc.relation.journalcode | J02249 | - |
dc.identifier.eissn | 1573-4978 | - |
dc.identifier.pmid | 12974468 | - |
dc.identifier.url | http://link.springer.com/article/10.1023/A:1024920418148 | - |
dc.subject.keyword | murine Hoxc8 gene | - |
dc.subject.keyword | proteom analysis | - |
dc.subject.keyword | putative Hoxc8 realizator genes | - |
dc.contributor.alternativeName | Kwon, Yunjeong | - |
dc.contributor.alternativeName | Kim, Myoung Hee | - |
dc.contributor.alternativeName | Kim, Byung Gyu | - |
dc.contributor.affiliatedAuthor | Kwon, Yunjeong | - |
dc.contributor.affiliatedAuthor | Kim, Myoung Hee | - |
dc.contributor.affiliatedAuthor | Kim, Byung Gyu | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 30 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 141 | - |
dc.citation.endPage | 148 | - |
dc.identifier.bibliographicCitation | MOLECULAR BIOLOGY REPORTS, Vol.30(3) : 141-148, 2003 | - |
dc.identifier.rimsid | 43900 | - |
dc.type.rims | ART | - |
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