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구강 편평세포암종 세포주에서 TGF-β1 type Ⅱ 수용체 발현

Other Titles
 Expression of the TGF-β1 type Ⅱ Receptor in Oral Squamous Cell Carcinoma Cell Lines 
 배경진  ;  최정희  ;  김진  ;  이은주  ;  김은정 
 Korean Journal of Oral and Maxillofacial Pathology (대한구강악안면병리학회지), Vol.27(1) : 25-35, 2003 
Journal Title
 Korean Journal of Oral and Maxillofacial Pathology (대한구강악안면병리학회지) 
Issue Date
Oral squamous cell carcinomas ; TGF-β1 ; TGF-β1 type Ⅱ receptor gene’s mutation
Oral squamous cell carcinomas ; TGF-β1 ; TGF-β1 type Ⅱ receptor gene’s mutation
The imbalance between epithelial cell growth and inhibitory factors may cause human epithelial cancer. The dysregulation of growth inhibitory effect of TGF-β1 has been recognized in a variety of carcinomas. This study aimed to investigate the expression of TGF-β1 type Ⅱ receptors(TβR-Ⅱ) in the carcinogenesis of squamous cell carcinoma(OSCC). Six cell lines established form OSCC in the department of Oral Pathology, Yonsei University College of Dentistry were used. DNA was extracted from harvested cells by phenol-chloroform method. Polymerase chain reaction (PCR) was done with each primer of exon 3, 4, 5, 6 of TβR-Ⅱ gene. PCR products were inserted to cloning vector (pGEM-T easy vector) and then analyzed to automatic DNA sequencing analyzer. Reverse trascriptase-PCR(RT-PCR) was performed to confirm the mRNA expression of TβR-Ⅱ gene. Western blotting was performed to detect the expression of the TβR-Ⅱ protein. As results, a frameshift within a polyadenine region of exon 3 was found in YD-8 cell line. In YD-17 cell line, a missense mutation at codon 238 of exon 4 was found, suggesting the alteration of amino acid from asparagine to aspartic acid. TβR-Ⅱ mRNA was detected in all cancer cell lines, but it was slightly decreased as compared to that of normal oral mucosal cells. In Western blotting, no TβR-Ⅱ protein was detected in all OSCC cell lines. These results suggested that the altered regulation of TGF-β1 function might play a role in the development of OSCC.
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2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jin(김진)
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