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Mycophenolic Acid와 Rapamycin 투여가 흰쥐 혈관평활근세포의 증식과 Collagen 생성에 미치는 영향

Other Titles
 Effect of Mycophenolic Acid and Rapamycin on the Proliferation and Collagen Synthesis of the Vascular Smooth Muscle Cell of Rat 
Authors
 박제현  ;  문장일  ;  서지연  ;  강상욱  ;  하헌주  ;  김혜진  ;  허규하  ;  김명수  ;  박기일  ;  김유선 
Citation
 Journal of the Korean Society for Transplantation (대한이식학회지), Vol.17(1) : 1-6, 2003 
Journal Title
 Journal of the Korean Society for Transplantation (대한이식학회지) 
ISSN
 1598-1711 
Issue Date
2003
Keywords
마이코페놀산 ; Mycophenolic acid ; 라파마이신 ; Rapamycin ; 혈관평활근세포 ; Vascular smooth muscle cell ; 증식 ; Proliferation ; 콜라겐 ; Collagen
Abstract
PURPOSE: Vascular smooth muscle cell (VSMC) proliferation and extracellular matrix protein accumulation play important roles in chronic allograft vasculopathy. Mycophenolic acid (MPA) or rapamycin (RPM) was reported to inhibit VSMC proliferation in vitro and in vivo. However, effects of MPA or RPM on collagen synthesis of VSMCs, and the combined effects of MPA and RPM treatment on VSMC proliferation are not yet reported. METHODS: VSMCs isolated from the aorta of Sprague-Dawley rats were cultured with EMEM supplemented with 10% fetal bovine serum and insulin/ transferrin. Growth arrested and synchronized cells were pretreated with test drugs (alone or combination of various concentrations of MPA and RPM) 1 hour before the addition of 10 ng/ml PDGF. Cell proliferation was assessed by [H(3)]- thymidine incorporation, and collagen synthesis by [H(3)]- proline incorporation. RESULTS: PDGF increased cell proliferation and collagen synthesis by 3.4- and 2.1-fold, respectively, compared to control. MPA at above 100 nM or RPM at above 1 nM effectively inhibited PDGF-induced cell proliferation and collagen synthesis. The IC(50) of MPA or RPM against PDGF-stimulated cell proliferation was between 100 nM and 1 micro M or between 1 nM and 10 nM, respectively. The combination of MPA and RPM showed additive effects on PDGF-induced VSMC proliferation in a multiple regression analysis (R(2)=0.508, P<0.05). CONCLUSION: The present study demonstrated that MPA or RPM significantly inhibited PDGF-induced VSMC proliferation. These independent phenomena were well maintained as suggested by additive effects after combination treatment. PDGF-induced collagen synthesis was also effectively suppressed by the treatment of MPA or RPM.
Files in This Item:
T200305868.pdf Download
DOI
OAK-2003-01068
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114228
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