Cited 0 times in
Inhibition of NF-κB Activation Increased Oxygen-Glucose Deprivation-Induced Cerebral Endothelial Cell Death
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김철훈 | - |
dc.date.accessioned | 2015-07-15T17:01:53Z | - |
dc.date.available | 2015-07-15T17:01:53Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114086 | - |
dc.description.abstract | INcreasing evidences suggest that ischemia-induced vascular damage in an integral step in the cascade of the cellular abd molecular events initiated by cerebral ischemia. In the present study, employing a mouse brain endothelioma-derived cell line, bEnd.3, and oxygen-glucose deprivation (OGD) as an in vitro stroke model, the role of nuclear factor kappa B(NF-κB) activation during ischemic injury was investigated. OGD was found to activate NF-κB and to induce bEnd.3 cell death in a time-dependent manner. OGD phosphorylated neither 32 Ser nor 42 Tyr of IκBα. OGD did not chage the amount of IκBα. The extents of OGD-induced cell death after 8 h, 10 h, 12 h and 14 h of OGD were 10%, 35%, 60% and 85%, respectively. Reperfusion following OGD did not cause additional cell death, indicating no reperfusion injury after ischemia insult in cerebral endothelial cells. There known as NF-κB inhibitors, including pyrrolidine dithiocarbamate (PDTC) plus zinc, aspirin and caffeic acid phenethyl ester (CAPE), inhibited OGD-induced NF-κB activation and increased OGD-induced bEnd.3 cell death in a dose dependent manner. there were no changes in the protein levels of bcl-2, bax and p53 which are modulated by NF-κB activity. these results suggest that NF-κB activation might be a protective mechanism for OGD-induced cell death in bEnd.3. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cerebral endothelial cell | - |
dc.subject.MESH | Oxygen-glucose deprivation | - |
dc.subject.MESH | Cell death | - |
dc.subject.MESH | Nuclear factor kappa B | - |
dc.title | Inhibition of NF-κB Activation Increased Oxygen-Glucose Deprivation-Induced Cerebral Endothelial Cell Death | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Lee Jinu | - |
dc.contributor.googleauthor | Kim Chul Hoon | - |
dc.contributor.googleauthor | Ahn Young Soo | - |
dc.contributor.googleauthor | Shim Kyu Dae | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01057 | - |
dc.relation.journalcode | J02104 | - |
dc.identifier.eissn | 2093-3827 | - |
dc.subject.keyword | Cerebral endothelial cell | - |
dc.subject.keyword | Oxygen-glucose deprivation | - |
dc.subject.keyword | Cell death | - |
dc.subject.keyword | Nuclear factor kappa B | - |
dc.contributor.alternativeName | Kim, Chul Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Chul Hoon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 7 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 65 | - |
dc.citation.endPage | 71 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, Vol.7(2) : 65-71, 2003 | - |
dc.identifier.rimsid | 55706 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.