수산화인회석을 근간으로 한 생체 활성 골시멘트의 연구

Abstract

Purpose: The purpose of this study is to propose a new bioactive bone cement (BBC) composed of bone powder (hydroxyapatite; HA), chitosan powder, and currently available polymethylmethacrylate (PMMA) bone cement for use in orthopaedic surgeries such as vertebroplasty or bone filler. Materials and Methods: Three types of proposed BBCs and a currently available commercial PMMA were tested. In vitro studies the surface morphology, chemical composition. changes in pH value along the time, exothermic temperatures, intrusion and cellular responses were investigated. SEM, radiological and histological examinations were performed in animal studies. Results: The major components of BBCs were C, 0, Ca, P, Cl, Si, S, Ba and Mg. The pH values in BBCs decreased after 1 day, however they eventually reached 7.2-7.4. The water absorbency, weight loss, and porosity in BBCs increased more than PMMA more than during degradation(p<0.05). However, the compressive Young's moduli and ultimate compressive strength (UCS) of BBCs were lower than those of PMMA(<0.05). The exothermic temperatures of the BBCs were considerably lower than that of PMMA (p<0.05). In view of setting time, it takes relatively longer for BBC Ⅱ and Ⅲ to be solidified than PMMA (p<0.05). The intrusion tests showed that the BBCs were more intrusive than PMMA (p<0.05). The cell proliferation test on BBC Ⅱ showed that the BBC Ⅱ was more preferable than the PMMA. No cytotoxic characteristics were found in all BBCs. In the animal test, BBC Ⅱ was more biocompatible as well as osteoconductible than the PMMA. Conclusion: The results of in vitro and animal studies indicated that the proposed BBCs have a potential of clinical application as replacement of the current PMMA bone cements.