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Effect of Ginseng Saponins on the Recombinant Serotonin Type 3A Receptor Expressed in Xenopus Oocytes: Implication of Possible Application as an Antiemetic

Authors
 Kyeong T. Min  ;  Bon N. Koo  ;  Zang-Hee Cho.  ;  Sung R. Ko  ;  Sun Joon Bai  ;  Jeong W. Kang 
Citation
 JOURNAL OF ALTERNATIVE AND COMPLEMENTARY MEDICINE, Vol.9(4) : 505-510, 2003 
Journal Title
 JOURNAL OF ALTERNATIVE AND COMPLEMENTARY MEDICINE 
ISSN
 1075-5535 
Issue Date
2003
MeSH
Animals ; Antiemetics/pharmacology* ; Antiemetics/therapeutic use ; Dose-Response Relationship, Drug ; Ginsenosides/pharmacology* ; Ginsenosides/therapeutic use ; Humans ; In Vitro Techniques ; Nausea/prevention & control ; Oocytes/metabolism* ; Panax* ; Patch-Clamp Techniques ; RNA, Messenger/biosynthesis ; Receptors, Serotonin, 5-HT3/drug effects* ; Receptors, Serotonin, 5-HT3/metabolism* ; Recombinant Proteins/drug effects ; Recombinant Proteins/metabolism ; Saponins/pharmacology ; Triterpenes/pharmacology ; Vomiting/prevention & control ; Xenopus laevis
Keywords
14499026
Abstract
Objectives: Nausea and vomiting are the most frequently reported side-effects by patients who are given general anesthesia perioperatively and patients with cancer who undergo chemotherapy or radiotherapy. Serotonin (5-hydroxytryptamine, 5HT) type 3A receptor (5HT3A receptor) is known to mediate nausea and vomiting and its antagonists have been used effectively to prevent and/or reduce the incidence and severity of nausea and vomiting. However, the adverse effects on cardiac function, such as QT interval prolongation, limit their routine use by these patients. This study was designed to elucidate the effect of ginseng saponins on the recombinant 5HT3A receptor expressed in the xenopus oocyte. Design: After in vitro transcription of the recombinant human 5HT3A receptor in the Xenopus laevis oocyte, we examined Panax ginseng saponins (total saponin [TS], panaxadiol saponin [PD] fraction, panaxatriol saponin [PT] fraction, and ginsenoside-Rb1 and -Rg1) for their ability to inhibit current flow through the 5HT3A receptor using the voltage-clamp technique. Results: All saponin fractions (TS, PD, PT fraction, as well as ginsenoside-Rb1 and -Rg1) inhibited the peak current induced by the agonist 5HT on the 5HT3A receptor in a concentration-dependent, reversible, and voltage-independent manner. The PT fraction inhibited 5HT-induced currents in 5HT3A receptor more than the PD fraction; meanwhile, there was a similar degree of inhibition between ginsenoside-Rg1 and -Rb1, the main substitutes of PT fraction and PD saponin fractions, respectively. Conclusions: These results indicate that ginseng saponins, especially PT fraction, have substantial inhibitory effects on the recombinant 5HT3A receptor, suggesting that some of the specific types of ginsenoside might have an antagonistic action against 5HT3A receptor related to nausea and vomiting.
Full Text
http://online.liebertpub.com/doi/abs/10.1089/107555303322284794
DOI
10.1089/107555303322284794
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Koo, Bon-Nyeo(구본녀) ORCID logo https://orcid.org/0000-0002-3189-1673
Min, Kyeong Tae(민경태) ORCID logo https://orcid.org/0000-0002-3299-4500
Bai, Sun Joon(배선준) ORCID logo https://orcid.org/0000-0001-5027-3232
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113881
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