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Natural Variation in Populations of Persistently Colonizing Bacteria Affect Human Host Cell Phenotype

Authors
 Rahul A. Aras  ;  Yongchan Lee  ;  Martin J. Blaser  ;  Richard M. Peek, Jr  ;  Dawn Israel  ;  Sung-Kook Kim 
Citation
 JOURNAL OF INFECTIOUS DISEASES, Vol.188(4) : 486-496, 2003 
Journal Title
JOURNAL OF INFECTIOUS DISEASES
ISSN
 0022-1899 
Issue Date
2003
MeSH
Amino Acid Sequence ; Antigens, Bacterial/chemistry ; Antigens, Bacterial/genetics* ; Apoptosis ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics* ; Base Sequence ; Cell Line ; Cell Size ; Coculture Techniques ; Gastric Mucosa/cytology* ; Gastric Mucosa/microbiology* ; Genetic Variation* ; Helicobacter pylori/genetics* ; Humans ; Interleukin-8/biosynthesis ; Molecular Sequence Data ; Phenotype ; Phosphorylation ; Recombination, Genetic/genetics ; Sequence Deletion/genetics ; Stomach/cytology ; Stomach/microbiology
Keywords
12898434
Abstract
The highly diverse bacterium Helicobacter pylori which persistently colonizes the human stomach, provides models to study the role of genome plasticity in host adaptation. Within H. pylori populations from 2 colonized individuals, intragenomic recombination between cagA DNA repeat sequences leads to deletion or duplication of tyrosine phosphorylation sites in the CagA protein, which is injected by a type IV secretion system into host cells. Experimental coculture of gastric epithelial cells with the strains containing these naturally occurring CagA phosphorylation site variants induced markedly divergent host cell morphologic responses. Mutants were constructed in which a phosphorylation site was either added or deleted in the expressed CagA protein; coculture studies confirmed that the naturally occurring differences in CagA phosphorylation are responsible for the observed phenotypic variation. These findings indicate that within an individual host, intragenomic recombination between H. pylori repetitive DNA produces strain variants differing in their signals to host cells
Files in This Item:
T200304412.pdf Download
DOI
10.1086/377098
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Yong Chan(이용찬) ORCID logo https://orcid.org/0000-0001-8800-6906
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113835
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