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Genetic analysis of the VP1 region of human enterovirus 71 strains isolated in Korea during 2000

Authors
 Y. M. Jee  ;  D.-S. Cheon  ;  H.-W. Cho  ;  J.-D. Yoon  ;  D.-S. Kim  ;  H. J. Chung  ;  E.-C. Kim  ;  J.-H. Lee  ;  K. S. Park  ;  S. H. Lee  ;  J. Lee  ;  Y.-S. Chung  ;  J. H. Cho  ;  K. Kim 
Citation
 ARCHIVES OF VIROLOGY, Vol.148(9) : 1735-1746, 2003 
Journal Title
 ARCHIVES OF VIROLOGY 
ISSN
 0304-8608 
Issue Date
2003
MeSH
Amino Acid Sequence ; Animals ; Capsid Proteins/chemistry ; Capsid Proteins/genetics* ; Cercopithecus aethiops ; Child ; Child, Preschool ; Enterovirus/classification ; Enterovirus/genetics* ; Female ; Fluorescent Antibody Technique ; Humans ; Infant ; Male ; Molecular Sequence Data ; Neutralization Tests ; Vero Cells
Keywords
Nucleotide ; Nucleotide Sequence ; Prime Pair ; Genomic Analysis ; Rhabdomyosarcoma
Abstract
We have isolated Human enterovirus 71 (EV71) from stool and CSF samples taken from patients with acute flaccid paralysis, herpangina, or hand, foot and mouth disease in 2000. Both the cell culture-neutralization test and RT-PCR were used to detect enteroviruses. Rhabdomyosarcoma (RD), HEP2c, and BGM cells were used for the isolation of viruses, and serotypes were determined by the neutralization test using EV71-specific antiserum. For genomic analysis, we amplified a 437-bp fragment of the 5'-noncoding region of the enterovirus genome and a 484-bp fragment of the VP3/VP1 region of EV71 by RT-PCR, with positive results. Products amplified using an EV71-specific primer pair were sequenced and compared with other isolates of EV71. Analysis of the nucleotide sequences of the amplified fragments showed that the EV71 isolates from patients were over 98% homologous and belonged to the genotype C.
Full Text
http://link.springer.com/article/10.1007%2Fs00705-003-0133-6
DOI
10.1007/s00705-003-0133-6
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Soo(김동수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113717
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