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말초신경 손상에 의한 배근신경절 세포의 전기생리적 특성 변화

Other Titles
 Changes of Electrophysiologic Properties of Dorsal Root Ganglion Cells in Peripheral Nerve-injured Rats 
Authors
 장성구  ;  정승수  ;  남택상  ;  윤덕미  ;  은정우  ;  임중우 
Citation
 KOREAN JOURNAL OF PAIN, Vol.16(1) : 23-32, 2003 
Journal Title
 KOREAN JOURNAL OF PAIN 
ISSN
 2005-9159 
Issue Date
2003
MeSH
dorsal root ganglion ; neuropathic pain ; norepinephrine ; patch clamp ; sympathetically maintained pain
Keywords
dorsal root ganglion ; neuropathic pain ; norepinephrine ; patch clamp ; sympathetically maintained pain
Abstract
Background: The neuropathic pain induced by peripheral nerve injury has been shown to be spontaneous, hyperalgesia and allodynia. If the neuropathic pain is related to the sympathetic system, it is classified as a sympathetically maintained pain (SMP). SMP is aggravated by sympathetic activation, and sympathetic block is used as a clinical treatment. Until now, it has been unclear what mechanism is involved in the aggravation of the pain due to sympathetic activation. Many investigations relating to this topic, using experimental animal models, have confirmed that the sensory nerve is activated due to sympathetic activation, and is mediated by the alpha adrenergic receptor. However, the detailed mechanism for the involvement of the alpha adrenergic receptor on the sympathetic-sensory coupling is controversial, as many previous experiments have been performed using in vivo preparations that could not exclude many complicating factors. Therefore, injured dorsal root ganglion cells were isolated, and the effects of norepinephrine, which is known to be involved in membrane excitability, investigated on several ionic channels. Methods: The neuropathic animal models were made by ligation of the L5 and L6 spinal nerves of rats. The dorsal root ganglion cells were immediately isolated, and the electrophysiologic properties studied using a patch clamp technique. Results: In the current clamp mode, the membrane excitability was confirmed to be increased by the norepinephrine, and in the voltage clamp mode, the inward Ca2⁢ and outward K⁢ currents were decreased by the norepinephrine, in the DRG neuron of the neuropathic model rat. Yohimbine, an α2 antagonist, suppressed the inhibitory effect of the norepinephrine on the inward Ca2⁢ and outward K⁢ currents of the neuropathic DRG neuron. Cadmium, a calcium channel blocker, suppressed the inhibitory effect of the norepinephrine on the outward K⁢ current, and iberiotoxin, a calcium activated potassium channel (KCa) blocker, suppressed the inhibitory effect of the norepinephrine on the outward K⁢ current of the neuropathic DRG neuron. Conclusions: These results suggest the direct actions of norepinephrine on DRG neuron at least contribute to the sympathetic-sensory coupling, and that suppression of the KCa channel activity may be an important mechanism in this process.
Files in This Item:
T200303331.pdf Download
DOI
OAK-2003-00378
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Nam, Taick Sang(남택상)
Chung, Seung Soo(정승수) ORCID logo https://orcid.org/0000-0002-3119-9628
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113538
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