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Microplate 를 이용한 장구균 동정법

Other Titles
 Identification of Enterococcus Species Using a Microplate 
Authors
 어영  ;  황규열  ;  이형환  ;  이경원  ;  윤갑준  ;  장인호 
Citation
 Journal of Laboratory Medicine and Quality Assurance (임상검사와 정도관리), Vol.25(2) : 215-220, 2003 
Journal Title
Journal of Laboratory Medicine and Quality Assurance(임상검사와 정도관리)
ISSN
 1225-097X 
Issue Date
2003
MeSH
Enterococcus ; Identification ; Microplate
Keywords
Enterococcus ; Identification ; Microplate
Abstract
Background : The aim of the study was to develop an accurate, convenient, and easy microplate system for the identification of enterococcal species from clinical specimens.

Methods : The microplate identification method was composed of twelve biochemical tests and identification programs. The tests comprised in microplate were initially screened by a two-tube method, NaCl-esculin hydrolysis and pyrrolidonyl-j3-naphthylamide test; arginine dihydrolase, acid production from mannitol, sorbitol, sucrose, arabinose, raffinose, methyl-a-D-glucopyranoside, and ribose in the microplate; and pigment production and hemolytic pattern in blood agar plate. The performance of the microplate for identifying enterococci to the species level was evaluated in comparison with conventional reference tests and commercial -kits.

Results : Among the 111 clinical isolates of Enterococcus species, the microplate system correctly identified 100% to genus level, and 91.0% to species level. All of E. casseliflavus, E durans, and EE hirae were correctly identified by the microplate. The diagnostic sensitivity and specificity for identification of Enterococcus species were as follows: 100% and 96.7% in EE

faecium, 93.5% and 100% in F faecalis, 100% and 97.2% in EE raffinosus, and 33.3% and 98.1%
in both F avium and E gallinarum.

Conclusions : It is concluded that the microplate method offers a simple, cost-effective, rapid,
and accurate identification system for the identification of most clinical isolates of Enterococcus species.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113382
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