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두부 손상이 골절 치유 촉진 시 동물 모델 및 유전자 발현 분석

Other Titles
 Animal Model and Gene Expression Analysis during the Accelerated Fracture Healing in Traumatic Brain Injury 
Authors
 김경희  ;  김학선  ;  문은수  ;  한수봉  ;  박희완  ;  이환모  ;  권언혜  ;  김향  ;  문성환 
Citation
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.6(2) : 177-185, 2003 
Journal Title
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지) 
ISSN
 1226-8933 
Issue Date
2003
MeSH
Traumatic brain injury ; Fracture healing ; cDNA microarray
Keywords
Traumatic brain injury ; Fracture healing ; cDNA microarray
Abstract
Purpose: This study was performed to create a traumatic brain injury (T131) animal model of up-regulated bone formation, and to show the possibility of comprehensive analysis of early gene expression from the hard tissue by cDNA microarray technique. Materials and Methods: Thirty Sprague-Dawley rats underwent either a severe brain injury operation pro¬cedure using the water pump with femur fracture (Group I, N=15), or femur frecture only (group II, N=15). The femur was nailed with a 20-gauge needle and fractured. The rats were euthanized at the day 1, 3, 8, 14, and 28 day. The volume of callus was calculated using GE PACS®. The total RNA was isolated from the 3rd day´ s callus and the gene expression was compared using microarray chips. Results: The average time until union was 28 days for control group and 14 days for ´1´BI group, which was significantly shorter. The volume of the callus (27.4 ± 8.5 mmz) in the TBI animals was clearly greater than that of the control group (13.0±6.3 mm?). The mRNA was successfully extracted from the callus and analysed using the c-DNA microarray, and. 312 genes were significantly increased and 227 genes were decreased in the TBI group. Conclusion: This study successfully made a model for a accelerated fracture healing in traumatic brain injury. We showed that cDNA microarray technique can be used to compare the expression of the mRNA in fracture callus. This method will be helpful in analysing the mechanism of accelerated fracture healing after brain injury.
Files in This Item:
T200302660.pdf Download
DOI
OAK-2003-00173
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hak Sun(김학선) ORCID logo https://orcid.org/0000-0002-8330-4688
Moon, Seong Hwan(문성환)
Moon, Eun Su(문은수)
Park, Hui Wan(박희완)
Lee, Hwan Mo(이환모) ORCID logo https://orcid.org/0000-0002-5405-3832
Hahn, Soo Bong(한수봉)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113333
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