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Cell adhesion-related gene expression by Helicobacter pylori in gastric epithelial AGS cells

DC FieldValueLanguage
dc.contributor.author김경환-
dc.date.accessioned2015-07-15T16:34:56Z-
dc.date.available2015-07-15T16:34:56Z-
dc.date.issued2003-
dc.identifier.issn1357-2725-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/113186-
dc.description.abstractHelicobacter pylori (H. pylori) infection leads to gastroduodenal inflammation, peptic ulceration and gastric carcinoma. H. pylori may induce disease-specific gene expression in gastric epithelial cells. cDNA microarray for 352 cancer-related genes was used to identify the genes altered by H. pylori (cagA positive) in gastric epithelial AGS cells. Expressions of the genes identified on the microarray and other genes closely associated with these genes were determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Western blot analysis and cell adhesion assay were performed to confirm the protein levels of the genes and the role of the genes on cell adhesion in H. pylori-infected AGS cells. As a result, the expression of four genes (galectin 1, aldolase A, integrin α5, LIM domain only 7 (LMO7)) were up-regulated by H. pylori in AGS cells, determined by cDNA microarray. RT-PCR analysis showed that the genes up-regulated by H. pylori were the genes regulating cell–cell adhesion and cell–extracellular matrix interaction, such as galectin-1 and galectin-3, integrin α5, and LIM domain only 7 (LMO7), and cancer-related glycolytic enzyme aldolase A and C. Cell adhesion to extracellular matrix proteins such as poly-l-lysine and fibronectin was mediated by H. pylori-induced expression of integrin α5. RT-PCR and Western blot analysis showed that E-cadherin, regulating cell adhesion and contact cell inhibition, was decreased by H. pylori in AGS cells. In conclusion, the increased expression of cell adhesion molecules and decrease in E-cadherin expression by H. pylori might contribute to cell adhesion, invasion and possibly cell proliferation in gastric epithelial cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1284~1296-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdenocarcinoma/etiology-
dc.subject.MESHAdenocarcinoma/genetics*-
dc.subject.MESHAdenocarcinoma/metabolism-
dc.subject.MESHAldehyde-Lyases/genetics-
dc.subject.MESHAldehyde-Lyases/metabolism-
dc.subject.MESHCadherins/genetics-
dc.subject.MESHCadherins/metabolism-
dc.subject.MESHCell Adhesion/genetics-
dc.subject.MESHDown-Regulation-
dc.subject.MESHEpithelial Cells/metabolism-
dc.subject.MESHEpithelial Cells/microbiology-
dc.subject.MESHGalectins/genetics-
dc.subject.MESHGalectins/metabolism-
dc.subject.MESHGastric Mucosa/metabolism-
dc.subject.MESHGastric Mucosa/microbiology-
dc.subject.MESHGene Expression Regulation-
dc.subject.MESHHelicobacter Infections/complications-
dc.subject.MESHHelicobacter Infections/genetics*-
dc.subject.MESHHelicobacter Infections/metabolism-
dc.subject.MESHHelicobacter pylori/genetics*-
dc.subject.MESHHomeodomain Proteins/genetics-
dc.subject.MESHHomeodomain Proteins/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHIntegrin alphaV/genetics-
dc.subject.MESHIntegrin alphaV/metabolism-
dc.subject.MESHLIM Domain Proteins-
dc.subject.MESHStomach Neoplasms/etiology-
dc.subject.MESHStomach Neoplasms/genetics*-
dc.subject.MESHStomach Neoplasms/metabolism-
dc.subject.MESHTranscription Factors/genetics-
dc.subject.MESHTranscription Factors/metabolism-
dc.subject.MESHTumor Cells, Cultured-
dc.subject.MESHUp-Regulation-
dc.titleCell adhesion-related gene expression by Helicobacter pylori in gastric epithelial AGS cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorJoo Weon Lim-
dc.contributor.googleauthorHyeyoung Kim-
dc.contributor.googleauthorKyung Hwan Kim-
dc.identifier.doiOAK-2003-00026-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00311-
dc.relation.journalcodeJ01089-
dc.identifier.eissn1878-5875-
dc.identifier.pmid12757765-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1357272503000517-
dc.subject.keyword12757765-
dc.contributor.alternativeNameKim, Kyung Hwan-
dc.contributor.affiliatedAuthorKim, Kyung Hwan-
dc.rights.accessRightsnot free-
dc.citation.volume35-
dc.citation.number8-
dc.citation.startPage1284-
dc.citation.endPage1296-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol.35(8) : 1284-1296, 2003-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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