Cited 0 times in
Adeno-associated virus mediated endostatin gene therapy in combination with topoisomerase inhibitor effectively controls liver tumor in mouse model
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 홍성이 | - |
dc.contributor.author | 김경섭 | - |
dc.contributor.author | 노성훈 | - |
dc.contributor.author | 노재경 | - |
dc.contributor.author | 이면희 | - |
dc.contributor.author | 정현철 | - |
dc.contributor.author | 최승호 | - |
dc.contributor.author | 형우진 | - |
dc.date.accessioned | 2015-07-14T17:32:42Z | - |
dc.date.available | 2015-07-14T17:32:42Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1007-9327 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113102 | - |
dc.description.abstract | AIM: rAAV mediated endostatin gene therapy has been examined as a new method for treating cancer. However, a sustained and high protein delivery is required to achieve the desired therapeutic effects. We evaluated the impact of topoisomerase inhibitors in rAAV delivered endostatin gene therapy in a liver tumor model. METHODS: rAAV containing endostatin expression cassettes were transduced into hepatoma cell lines. To test whether the topoisomerase inhibitor pretreatment increased the expression of endostatin, Western blotting and ELISA were performed. The biologic activity of endostatin was confirmed by endothelial cell proliferation and tube formation assays. The anti-tumor effects of the rAAV-endostatin vector combined with a topoisomerase inhibitor, etoposide, were evaluated in a mouse liver tumor model. RESULTS: Topoisomerase inhibitors, including camptothecin and etoposide, were found to increase the endostatin expression level in vitro. The over-expressed endostatin, as a result of pretreatment with a topoisomerase inhibitor, was also biologically active. In animal experiments, the combined therapy of topoisomerase inhibitor, etoposide with the rAAV-endostatin vector had the best tumor-suppressive effect and tumor foci were barely observed in livers of the treated mice. Pretreatment with an etoposide increased the level of endostatin in the liver and serum of rAAV-endostatin treated mice. Finally, the mice treated with rAAV-endostatin in combination with etoposide showed the longest survival among the experimental models. CONCLUSION: rAAV delivered endostatin gene therapy in combination with a topoisomerase inhibitor pretreatment is an effective modality for anticancer gene therapy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | WORLD JOURNAL OF GASTROENTEROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae/genetics* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antineoplastic Agents/pharmacology | - |
dc.subject.MESH | Antineoplastic Agents, Phytogenic/pharmacology | - |
dc.subject.MESH | Camptothecin/pharmacology* | - |
dc.subject.MESH | Carcinoma, Hepatocellular/drug therapy* | - |
dc.subject.MESH | Carcinoma, Hepatocellular/mortality | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Combined Modality Therapy | - |
dc.subject.MESH | Drug Synergism | - |
dc.subject.MESH | Endostatins/pharmacology | - |
dc.subject.MESH | Endothelium, Vascular/cytology | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology* | - |
dc.subject.MESH | Etoposide/pharmacology | - |
dc.subject.MESH | Gene Expression/drug effects | - |
dc.subject.MESH | Genetic Therapy/methods* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Liver Neoplasms/drug therapy* | - |
dc.subject.MESH | Liver Neoplasms/mortality | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Sarcoma/drug therapy* | - |
dc.subject.MESH | Sarcoma/mortality | - |
dc.subject.MESH | Survival Rate | - |
dc.subject.MESH | Topoisomerase Inhibitors | - |
dc.subject.MESH | Umbilical Veins/cytology | - |
dc.title | Adeno-associated virus mediated endostatin gene therapy in combination with topoisomerase inhibitor effectively controls liver tumor in mouse model | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Sung Yi Hong | - |
dc.contributor.googleauthor | Myun Hee Lee | - |
dc.contributor.googleauthor | Seung Ho Choi | - |
dc.contributor.googleauthor | Sung Hoon Noh | - |
dc.contributor.googleauthor | Woo Jin Hyung | - |
dc.contributor.googleauthor | Jae Kyung Roh | - |
dc.contributor.googleauthor | Hyun Cheol Jung | - |
dc.contributor.googleauthor | Kyung Sup Kim | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04401 | - |
dc.contributor.localId | A00297 | - |
dc.contributor.localId | A01281 | - |
dc.contributor.localId | A01290 | - |
dc.contributor.localId | A02749 | - |
dc.contributor.localId | A03773 | - |
dc.contributor.localId | A04382 | - |
dc.contributor.localId | A04102 | - |
dc.relation.journalcode | J02795 | - |
dc.identifier.eissn | 2219-2840 | - |
dc.identifier.pmid | 15069724 | - |
dc.contributor.alternativeName | Hong, Sung Yi | - |
dc.contributor.alternativeName | Kim, Kyung Sup | - |
dc.contributor.alternativeName | Noh, Sung Hoon | - |
dc.contributor.alternativeName | Roh, Jae Kyung | - |
dc.contributor.alternativeName | Lee, Myun Hee | - |
dc.contributor.alternativeName | Chung, Hyun Cheol | - |
dc.contributor.alternativeName | Choi, Seung Ho | - |
dc.contributor.alternativeName | Hyung, Woo Jin | - |
dc.contributor.affiliatedAuthor | Hong, Sung Yi | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Sup | - |
dc.contributor.affiliatedAuthor | Noh, Sung Hoon | - |
dc.contributor.affiliatedAuthor | Roh, Jae Kyung | - |
dc.contributor.affiliatedAuthor | Lee, Myun Hee | - |
dc.contributor.affiliatedAuthor | Chung, Hyun Cheol | - |
dc.contributor.affiliatedAuthor | Hyung, Woo Jin | - |
dc.contributor.affiliatedAuthor | Choi, Seung Ho | - |
dc.rights.accessRights | not available | - |
dc.citation.volume | 10 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1191 | - |
dc.citation.endPage | 1197 | - |
dc.identifier.bibliographicCitation | WORLD JOURNAL OF GASTROENTEROLOGY, Vol.10(8) : 1191-1197, 2004 | - |
dc.identifier.rimsid | 56816 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.