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The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.

Authors
 Young-Sun Kang  ;  Jae Y. Kim  ;  Chae Gyu Park  ;  Ralph M. Steinman  ;  Jutta M. Loeffler  ;  Thomas M. Moran  ;  Alla Pritsker  ;  Anna Charalambous  ;  Maggi Pack  ;  Sandra A. Bruening 
Citation
 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.101(1) : 215-220, 2004 
Journal Title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN
 0027-8424 
Issue Date
2004
MeSH
Amino Acid Sequence ; Animals ; Antibodies, Monoclonal/administration & dosage ; Antibodies, Monoclonal/isolation & purification ; CHO Cells ; Cricetinae ; Down-Regulation ; Lectins, C-Type/antagonists & inhibitors ; Lectins, C-Type/genetics ; Lectins, C-Type/metabolism* ; Mice ; Mice, Inbred C57BL ; Molecular Sequence Data ; Phagocytosis ; Polysaccharides, Bacterial/classification ; Polysaccharides, Bacterial/immunology* ; Serotyping ; Spleen/immunology* ; Spleen/microbiology* ; Streptococcus pneumoniae/classification ; Streptococcus pneumoniae/immunology* ; Streptococcus pneumoniae/pathogenicity ; Transfection
Abstract
SIGN-R1, a recently discovered C-type lectin expressed at high levels on macrophages within the marginal zone of the spleen, mediates the uptake of dextran polysaccharides by these phagocytes. We now find that encapsulated Streptococcus pneumoniae are rapidly cleared by these macrophages from the bloodstream, and that capture also takes place when different cell lines express SIGN-R1 after transfection. To assess the role of the capsular polysaccharide of S. pneumoniae (CPS) in the interaction of SIGN-R1 with pneumococci, we first studied binding and uptake of serotype 14 CPS in transfected cells. Binding was observed and was of a much higher avidity (3,000-fold) for CPS 14 than dextran. The CPSs from four different serotypes were also cleared by marginal zone macrophages in vivo. To establish a role for SIGN-R1 in this uptake, we selectively down-regulated expression of the lectin by pretreatment of the mice with SIGN-R1 antibodies, including a newly generated hamster monoclonal called 22D1. For several days after this transient knockout, the marginal zone macrophages were unable to take up either CPSs or dextrans. Therefore, marginal zone macrophages in mice have a receptor that interacts with capsular pneumococcal polysaccharides, setting the stage for further studies of the functional consequences of this interaction.
Files in This Item:
T200404853.pdf Download
DOI
10.1073/pnas.0307124101
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Park, Chae Gyu(박채규) ORCID logo https://orcid.org/0000-0003-1906-1308
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113010
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