298 412

Cited 0 times in

무모 생쥐에서 vinyl carbamate epoxide와 TPA로 유발된 종양과 비종양 병변의 표피지질 및 칼슘의 변화

Other Titles
 The Changes of Epidermal Lipid and Calcium in the Lesion of Skin Tumor and Non-tumor of Hairless Mice Induced by Vinyl Carbamate Epoxide and TPA. 
Authors
 안성구  ;  박하나  ;  이승헌  ;  이상주  ;  최응호  ;  전수영  ;  천승현 
Citation
 Korean Journal of Dermatology (대한피부과학회지), Vol.42(10) : 1304-1312, 2004 
Journal Title
Korean Journal of Dermatology(대한피부과학회지)
ISSN
 0494-4739 
Issue Date
2004
Keywords
Vinyl carbamate epoxide ; Skin tumor or non-skin tumor ; TPA ; Calcium ; Epidermal lipid
Abstract
Background: Chemically induced epidermal carcinogenesis is usually divided into two stages, the initiation and promotion. The initiation involves conversion of some epidermal cells into latent neoplastic cells and the promotion is proliferation of the transformed cells. Ethyl carbamate (EC) has been identified at low microgram quantities in various fermented beverages, distilled products and tobacco smoke. It has been known as a initiator of tumor. Oxidation of the ethyl group of EC is followed by dehydration to yield the carcinogen vinyl carbamate (VC). This is further oxidized to vinyl carbamate epoxide (VCO). VC and VCO proved to be much more carcinogenic than EC.

Object: This study is attemped to investigate the skin tumor and non-skin tumor in hairless mice induced by application of 12-0-tetradecanoyl-phorbol-13-acetate (TPA) on the skin initiated with VCO and its relationship with calcium gradient and epidermal lipid.

Methods: In this experiment, the tumor induction was performed by painting the mouse skin once a week for five weeks with VCO solution, and then 12-0-tetradecanoyl-phobol-13-acetate (TPA) was treated in the same manner twice a week for 40 weeks. We biopsied the skin at 5, 10, 25, 30, 35 and 40 weeks and stained the specimens with hematoxylin-eosin, Ru04 postfixation and ion capture cytochemistry for calcium staining.

Results: The results are summerized as follows1. Cellular proliferation, hyperkeratosis and dysplasia of the epidermis were more prominent in skin tumors than non-skin tumors. Papillomas were developed at 8 weeks after application of VCO- TPA but not TPA alone. The occurrence of keratoacanthoma and squamous cell carcinoma was 33 and 39 weeks, respectively.2. Calcium gradient was distorted in the only TPA treatment group but normal in the control group. Calcium deposition was increased through all layers of epidermis and the calcium gradient was disappeared in the epidermis of tumors in the VCO-TPA treatment group. These findings were similar to papilloma, keratoacanthoma and squamous cell carcinoma.3. Fragmented, incomplete lipid bilayer formation, dilated intercellular spaces and multiple lacunar domains were prominent in the VCO-TPA and TPA treatment groups but not in the control group. The VCO-TPA treatment group has shown more epidermal lipid damage than that of the only TPA treatment group.4. Diploid DNA histogram patterns were observed in all the control and TPA treatment groups. But aneuploidy was observed in 1 of 3 keratoacanthomas and 3 of 3 squamous cell carcinomas.

Conclusion: From the above results, it is concluded that various skin tumors, such as papilloma, keratoacanthoma and squamous cell carcinoma or non-skin tumor were produced by VCO. Skin tumors showed various, distinctive light microscopic or electron microscopic changes compared to the non-skin tumor. It is thought that intercellular lipid change and calcium gradient disappearance in the epidermis have an important role in the carcinogenesis.
Files in This Item:
T200404099.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Seung Hun(이승헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/112900
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links