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Analysis of amino acid sequence variations and immunoglobulin E-binding epitopes of German cockroach tropomyosin
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 용태순 | - |
dc.contributor.author | 이인용 | - |
dc.contributor.author | 이종원 | - |
dc.contributor.author | 이한일 | - |
dc.contributor.author | 정경영 | - |
dc.contributor.author | 홍천수 | - |
dc.contributor.author | 정경용 | - |
dc.date.accessioned | 2015-07-14T17:26:21Z | - |
dc.date.available | 2015-07-14T17:26:21Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1071-412X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/112883 | - |
dc.description.abstract | The allergenicities of tropomyosins from different organisms have been reported to vary. The cDNA encoding German cockroach tropomyosin (Bla g 7) was isolated, expressed, and characterized previously. In the present study, the amino acid sequence variations in German cockroach tropomyosin were analyzed in order to investigate its influence on allergenicity. We also undertook the identification of immunodominant peptides containing immunoglobulin E (IgE) epitopes which may facilitate the development of diagnostic and immunotherapeutic strategies based on the recombinant proteins. Two-dimensional gel electrophoresis and immunoblot analysis with mouse anti-recombinant German cockroach tropomyosin serum was performed to investigate the isoforms at the protein level. Reverse transcriptase PCR (RT-PCR) was applied to examine the sequence diversity. Eleven different variants of the deduced amino acid sequences were identified by RT-PCR. German cockroach tropomyosin has only minor sequence variations that did not seem to affect its allergenicity significantly. These results support the molecular basis underlying the cross-reactivities of arthropod tropomyosins. Recombinant fragments were also generated by PCR, and IgE-binding epitopes were assessed by enzyme-linked immunosorbent assay. Sera from seven patients revealed heterogeneous IgE-binding responses. This study demonstrates multiple IgE-binding epitope regions in a single molecule, suggesting that full-length tropomyosin should be used for the development of diagnostic and therapeutic reagents. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 874~878 | - |
dc.relation.isPartOf | CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Alternative Splicing* | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigen-Antibody Reactions | - |
dc.subject.MESH | Cockroaches/immunology* | - |
dc.subject.MESH | Cross Reactions/genetics | - |
dc.subject.MESH | Cross Reactions/immunology | - |
dc.subject.MESH | DNA, Complementary | - |
dc.subject.MESH | Immunodominant Epitopes/genetics* | - |
dc.subject.MESH | Immunoglobulin E/immunology* | - |
dc.subject.MESH | Protein Isoforms | - |
dc.subject.MESH | Sequence Analysis, Protein* | - |
dc.subject.MESH | Tropomyosin/genetics | - |
dc.subject.MESH | Tropomyosin/immunology* | - |
dc.title | Analysis of amino acid sequence variations and immunoglobulin E-binding epitopes of German cockroach tropomyosin | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Thoracic & Cardiovascular Surgery (흉부외과학) | - |
dc.contributor.googleauthor | Kyoung Yong Jeong | - |
dc.contributor.googleauthor | Jongweon Lee | - |
dc.contributor.googleauthor | Tai-Soon Yong | - |
dc.contributor.googleauthor | Chein-Soo Hong | - |
dc.contributor.googleauthor | Han-Il Ree | - |
dc.contributor.googleauthor | In-Yong Lee | - |
dc.identifier.doi | 10.1128/CDLI.11.5.874-878.2004 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02424 | - |
dc.contributor.localId | A03059 | - |
dc.contributor.localId | A03144 | - |
dc.contributor.localId | A03279 | - |
dc.contributor.localId | A04448-1 | - |
dc.contributor.localId | A03572 | - |
dc.relation.journalcode | J00547 | - |
dc.identifier.eissn | 1098-6588 | - |
dc.identifier.pmid | 15358646 | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.alternativeName | Lee, In Yong | - |
dc.contributor.alternativeName | Lee, Jong Weon | - |
dc.contributor.alternativeName | Ree, Han Il | - |
dc.contributor.alternativeName | Chung, Kyung Young | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Lee, In Yong | - |
dc.contributor.affiliatedAuthor | Lee, Jong Weon | - |
dc.contributor.affiliatedAuthor | Ree, Han Il | - |
dc.contributor.affiliatedAuthor | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Jeong, Kyoung Yong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 11 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 874 | - |
dc.citation.endPage | 878 | - |
dc.identifier.bibliographicCitation | CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY , Vol.11(5) : 874-878, 2004 | - |
dc.identifier.rimsid | 36771 | - |
dc.type.rims | ART | - |
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