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Characterization of antihypertensive angiotensin I-converting enzyme inhibitor from Saccharomyces cerevisiae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이대형 | - |
dc.date.accessioned | 2015-07-14T17:25:16Z | - |
dc.date.available | 2015-07-14T17:25:16Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/112847 | - |
dc.description.abstract | This study describes the purification and characterization of a novel antihypertensive angiotensin I-converting enzyme (ACE) inhibitory peptide from Saccharomyces cerevisiae. Maximal production of the ACE inhibitor from Saccharomyces cerevisiae was obtained from 24 h of cultivation at 30°C and its ACE inhibitory activity was increased by about 1.5 times after treatment of the cell-free extract with pepsin. After the purification of ACE inhibitory peptides with ultrafiltration, Sephadex G-25 column chromatography, and reverse-phase HPLC, an active fraction with an IC50 of 0.07 mg and 3.5% yield was obtained. The purified peptide was a novel decapeptide, showing very low similarity to other ACE inhibitory peptide sequences, and its amino acid sequence was Tyr-Asp-Gly-Gly-Val-Phe-Arg-Val-Tyr-Thr. The purified inhibitor competitively inhibited ACE and also showed a clear antihypertensive effect in spontaneously hypertensive rats (SHR) at a dosage of 1 mg/kg body weight | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1318~1323 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Characterization of antihypertensive angiotensin I-converting enzyme inhibitor from Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | KIM, Jae-Ho | - |
dc.contributor.googleauthor | Dae-Hyoung LEE | - |
dc.contributor.googleauthor | Jong-Soo LEE | - |
dc.contributor.googleauthor | Kun-Sub CHUNG | - |
dc.contributor.googleauthor | Seoung-Chan JEONG | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02714 | - |
dc.relation.journalcode | J01594 | - |
dc.identifier.eissn | 1738-8872 | - |
dc.subject.keyword | Antihypertension | - |
dc.subject.keyword | Saccharomyces cerevisiae | - |
dc.subject.keyword | angiotensin I-converting enzyme inhibitor | - |
dc.contributor.alternativeName | Lee, Dae Hyoung | - |
dc.contributor.affiliatedAuthor | Lee, Dae Hyoung | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 14 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1318 | - |
dc.citation.endPage | 1323 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Vol.14(6) : 1318-1323, 2004 | - |
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