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위장관 감작을 통한 땅콩 알레르기의 생쥐모델 확립

Other Titles
 Development of Murine Model of Peanut Allergy by Intragastric Sensitization of Peanut Extract 
 이수영  ;  박석원  ;  김규언  ;  손명현  ;  염혜영  ;  오세조 
 Journal of Asthma, Allergy and Clinical Immunology (천식 및 알레르기), Vol.24(4) : 428-434, 2004 
Journal Title
 Journal of Asthma, Allergy and Clinical Immunology (천식 및 알레르기) 
Issue Date
Peanut allergy ; Murine model ; Intragastric sensitization
BACKGROUND: Peanut allergy has been increased in westernized countries and recently it is increasing in Korea, and the peanut allergy is one of the most potent food allergies, even though the prevalence is not high compared to other food allergies. As like other food allergies, allergen avoidance is the only therapeutic option for peanut allergy. For the mechanistic or immunologic studies of food allergy is very limited in human, so we need a good quality of animal model of food allergy. OBJECTIVE: The purpose of this study was to develop a murine model of IgE mediated peanut allergy which mimics human peanut allergy. METHOD: C3H/HeJ mice were sensitized intragastricly, at day 1, 2, 3, 7, 21 with 1 mg (Group I) or 5 mg (Group II) of crude peanut extract mixed with cholera toxin, or sensitized by intraperitoneally (1 mg, Group III) at day 1, 7, 21 with alum solution, and the Group IV mice were served as naive control. Mice were challenged intragastricly at week 5. Serum peanut specific IgE, IgG1 were measured atweekly interval, and the splenocyte proliferation assay and cytokine profiles were evaluated after challenge at week 5. RESULT: Peanut specific IgE levels markedly increased at week 3, and the IgE responses increased till week 5 in intragastricly sensitized mice (Group I and II), while the initial profound IgE responses were abrogated after week 3 in intraperitoneally sensitized mice (Group III). The IgE levels were higher in Group II compared to Group I mice, and the IgE levels were consistent with fecal peanut specific IgA levels and anaphylaxis symptom score at week 5 challenge. Peanut stimulated productions of IL-4 and INF-gamma were higher in Group I and II compared to Group III. CONCLUSION: In this experiment, we have established relatively good quality of murine model of food allergy by intragastric sensitization which showed proper peanut specific IgE responses and cytokine productions. We suggest that this model could provide a good animal model for future research in food allergy.
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1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyu Earn(김규언)
Sohn, Myung Hyun(손명현) ORCID logo https://orcid.org/0000-0002-2478-487X
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